血清抗-FH、GP73、IFI16在自身免疫性肝炎中水平及与肝组织炎症活动的相关性

Serum Anti- FH,GP73,IFI16 Levels in Autoimmune Hepatitis and Their Correlations with Hepatic Inflammatory Activities

目的 探究血清抗延胡索酸水合酶抗体(抗-FH)、高尔基体跨膜糖蛋白73(GP73)、干扰素γ诱导蛋白16(IFI16)在自身免疫性肝炎中的水平,明确血清标志物是否可以与肝组织炎症活动具有相关性。方法 选取2016年10月至2022年6月我院门诊就诊的自身免疫性肝炎患者100例,另入组因外伤入院的健康对照100例,通过免疫组织化学法和ELISA法分析肝组织和血清中抗-FH、GP73、IFI16水平,分析外周血自身免疫相关炎症因子白细胞介素1(IL-1)、白细胞介素2(IL-2)、白细胞介素6(IL-6)、白细胞介素8(IL-8)、白细胞介素10(IL-10)、白细胞介素17(IL-17),以及自身免疫相关炎性趋化因子C-C Motif趋化因子配体2(CCL2)、C-X-C Motif趋化因子配体2(CXCL2)和C-X-C Motif趋化因子配体10(CXCL10)水平,并统计血清中抗-FH、GP73、IFI16与各炎症指标的相关性,同时通过受试者工作曲线分析血清中抗-FH、GP73、IFI16对组织炎症反应的预测效能。结果 与对照组相比,自身免疫性肝炎患者组织和血清抗-FH、GP73、IFI16水平升高,且自身免疫相关炎症因子IL-1、IL2、IL-6、IL-8、IL-10、IL17,以及自身免疫相关炎性趋化因子CCL2、CXCL2和CXCL10水平升高,抗-FH与血清炎症因子IL-1、IL2、IL-6、IL-8、IL-10、IL17,以及炎性趋化因子CCL2、CXCL2和CXCL10呈显著的正相关关系,GP73与血清炎症因子IL-1、IL2、IL-6、IL-8、IL-10、IL17,以及炎性趋化因子CCL2、CXCL2和CXCL10呈显著的的正相关关系,IFI16血清炎症因子IL-1、IL2、IL-6、IL-8、IL-10、IL17,以及炎性趋化因子CXCL2和CXCL10呈显著的正相关关系(P<0.05),抗-FH、GP73、IFI16均可以有效地预测肝组织炎症活动,且三者联合使用效力最高。结论 自身免疫性肝炎患者血清抗-FH、GP73、IFI16水平升高,并且与肝组织炎症活动强度呈正相关,三者可以有效地预测肝组织炎症活动。

Objective To explore expression levels of serum anti-fumarate hydratase antibody(anti-FH),Golgi transmembrane glycoprotein 73(GP73)and interferonγ,and inducible protein 16(IFI16)in autoimmune hepatitis and whether serum markers can reflect the intensity of liver tissue inflammation.Methods From October,2016 to June,2022,100 patients with autoimmune hepatitis who were admitted as outpatients in our hospital and 100 healthy controls who were admitted due to trauma were enrolled for the study.The expression levels of anti-FH,GP73,IFI16 in liver tissue and serum were analyzed by immunohistochemistry and ELISA methods,and the expression levels of inflammatory factors IL-1,IL-2,IL-6,IL-8,IL-10,IL-17 in peripheral blood,as well as inflammatory chemokines CCL2,CXCL2,and CXCL10 in serum were analyzed.The correlations between GP73,IFI16 and various inflammatory indicators were evaluated.The predictive efficacies of anti-FH,GP73,and IFI16 in serum and tissue inflammatory reaction were analyzed through the subject work curve.Results Compared with the control group,the expression levels of anti-FH,GP73,IFI16 in tissues and serum of patients with autoimmune hepatitis were increased,and the expression levels of inflammatory factors IL-1,IL-2,IL-6,IL-8,IL-10,IL-17,and inflammatory chemokines CCL2,CXCL2,and CXCL10 were also increased.Anti-FH was positively correlated with serum inflammatory factors IL-1,IL-2,IL-6,IL-8,IL-10,and IL-17,as well as inflammatory chemokines CCL2,CXCL2,and CXCL10.GP73 had a significant positive correlation with serum inflammatory factors IL-1,IL-2,IL-6,IL-8,IL-10,IL-17,and inflammatory chemokines CCL2,CXCL2,and CXCL10.IFI16 had a significant positive correlation with serum inflammatory factors IL-1,IL-2,IL-6,IL-8,IL-10,and IL-17,and inflammatory chemokines CXCL2 and CXCL10(P<0.05).Anti-FH,GP73,and IFI16 could effectively predict liver inflammation,and their combined use had the highest efficacy.Conclusion The high expression of serum anti-FH,GP73 and IFI16 in patients with autoimmune hepatitis can reflect the intensity of liver tissue inflammation,and the combined three test can effectively predict liver tissue inflammation.