番木瓜赖氨酸基序类受体激酶LysM-RLK基因家族鉴定及生物信息学分析

Identification and Bioinformatics Analysis of Lysine-like Receptor Kinase LysM-RLK Gene Family Based on the Whole Genome of Papaya

本研究采用生物信息学方法在番木瓜全基因组范围系统鉴定了7个LysM-RLK家族基因,并解析了其染色体分布特征、基因结构特征、蛋白理化性质和亚细胞定位信息。基因家族进化分析表明番木瓜的7个LysM-RLK基因可划分为3个亚组(Lym-II,Lym-III,Lym-IV),并分布于4条染色体上。基因复制是番木瓜LysM-RLK家族进化的主要驱动力,基因复制分析鉴定得到7对复制基因。启动子序列调控元件分析发现其包含多个光响应元件及参与低温应答和逆境相关激素信号应答的元件。大规模比较转录组分析系统地解析了LysM-RLK在番木瓜生物胁迫、非生物胁迫条件下及不同组织中的基因表达模式,揭示其特异性的表达模式及潜在的生物学功能。复制基因对的表达分化、结构域组成差异和蛋白质三级结构的变化,暗示其在进化过程中的亚功能化现象。生物和非生物胁迫条件下的核心共表达网络分析鉴定了27个与LysM-RLK3强关联的核心基因,揭示了LysM-RLK3潜在的转录调控模式。相关结果为番木瓜LysM-RLK家族起源演化、功能分化及其在逆境响应和生长发育过程中的角色提供重要依据。

In this study,seven LysM-RLK subfamily genes were systematically identified in the whole genome of pa-paya using bioinformatics methods,and the chromosome distribution,gene structure,protein physical and chemical properties,and subcellular localization information were analyzed.Familial evolutionary analysis showed that papaya LysM-RLK could be further divided into three subgroups(Lym-II,Lym-III,Lym-IV),distributed on four chromosomes.Recent gene replication is the main driver of the evolution of the papaya LysM-RLK subfamily.The analysis of promoter sequence regulatory elements found that it contained multiple photoresponse elements and elements involved in low temperature response and stress related hormone signal response.Large-scale comparative transcriptome analysis systematically analyzed the gene expression patterns of LysM-RLK under papaya biostress and abiotic stress and different tissues,revealing its specific expression patterns and potential biological functions.The expression differentiation of replicating gene pairs,differences in domain composition,and changes in the tertiary structure of proteins suggest subfunctionalization in the process of evolution.Core co-expression network analysis under biotic and abiotic stress conditions identified 27 core genes strongly associated with LysM-RLK3,revealing the potential transcriptional regulatory patterns of LysM-RLK3.The results would provide important insights into the evolution,functional differentiation and role of the papaya LysM-RLK subfamily in adversity response and growth and development.