朱顶红曼谷玫瑰愈伤组织途径再生体系的建立

Establishment of Callus Regeneration System of Hippeastrum‘Bangkok Rose’

朱顶红是我国近年来从国外大量引种并进行推广的新潮花卉,但其种苗昂贵,常规的鳞片扦插繁殖速度慢且需要消耗大量的种球;同时,朱顶红的新品种培育常采用杂交育种,定向性较差。为建立朱顶红的高效再生体系,促进种球工厂化生产并进行定向育种,以朱顶红曼谷玫瑰(Hippeastrum‘Bankkok Rose’)无菌苗叶片基部为外植体,建立愈伤组织再生体系,探究不同植物生长调节剂对愈伤组织的诱导、增殖、分化及生根移栽的影响。结果表明:将无菌苗叶片基部置于添加2,4-D 2.00 mg/L+TDZ 0.50 mg/L的MS培养基上培养45 d,愈伤组织诱导率最高,为39.67%。愈伤组织增殖的最佳培养基为MS+6-BA 2.00 mg/L,平均每20 d增殖4.01倍。愈伤组织分化的最适培养基为MS+KT 0.50 mg/L,60 d时不定芽分化系数达10.59,成苗系数达5.67。在MS+IBA 0.50 mg/L的培养基中进行生根培养,30 d生根率达100%。将生根培养30 d的小植株移栽至椰糠∶泥炭土∶蛭石=1∶1∶1的基质中培养,30 d后存活率达93.33%。本研究能为朱顶红种苗的工厂化繁殖提供技术支撑,也可为其后续的分子育种提供优良的受体材料。

Hippeastrum is a new kind of flower introduced in large scale from abroad in recent years,but its seedlings are expensive,regular scale cuttage propagation speed is slow and needs a large number of mother bulbs.Cross breeding,a common method for its new varieties,however,has poor orientation.This aim of this study was to establish an efficient callus regeneration system for rapid propagation and factory production of seedlings,which can also be used for the orientation breeding of Hippeastrum.The effects about different plant growth regulators on callus induction and plantlet regeneration were studied with the leaves of Hippeastrum‘Bankkok Rose’plantlet in vitro.The highest induction rate of callus(39.67%)was observed when the basal leaves of the buds in vitro were cultured on MS+2,4-D 2.00 mg/L+TDZ 0.50 mg/L medium for 45 days.The optimal medium for callus proliferation was MS+6-BA 2.00 mg/L,and the average proliferation was 4.01 times every 20 days.The optimal medium for callus differentiation was MS+KT 0.50 mg/L.After 60 days,the adventitious bud differentiation coefficient reached 10.59,and the seedling formation coefficient was 5.67.The rooting rate reached 100%after 30 days in MS+IBA 0.50 mg/L.After 30 days of root culture,the small plants were transplanted to the coir:peat soil:vermiculite=1:1:1 substrate,and the survival rate reached 93.33%after 30 days.This study could provide technical support for industrial propagation of Hippeastrum seedlings,and also provide excellent receptor materials for subsequent molecular breeding.