潜在马铃薯晚疫病抗性基因的表达和SNP标记分析

Expression and SNP Analysis of Candidate Solanum tuberosum Phytophthora infestans Resistance Genes

从马铃薯参考基因组中筛选出了433个NBS-LRR类基因,并绘制这些基因在基因组中的分布图,这些基因的其中一部分成簇分布。马铃薯基因组测序协会(PGSC)的转录组数据分析显示,只有12个NBS-LRR类基因在PGSC的转录组数据中完全没有检测到表达,不同NBS-LRR类基因转录水平不同,一部分NBS-LRR类基因有较高的生物胁迫和激素刺激应激性。分析了这433个基因的编码区域的单核苷酸多态性(SNP)位点,SNP标记作为在高通量测序和生物大数据分析中最常使用的遗传标记,有助于鉴定NBS-LRR基因在不同马铃薯品种中的实际抗病性。

In this paper,433 NBS-LR R genes were screened from the Solanum tuberosum reference genome,and the distribution of these genes in the genome were mapped,showing that some of these genes were distributed in clusters.Only 12 NBS-LRR genes were not detected in all of the transcriptome analysis data of PGSC.The expression levels of different NBS-LRR genes were different,and some NBS-LRR genes had higher biological and hormonal stimulation irritability.The single nucleotide polymorphism(SNP)sites in the coding regions of these 433 genes were also analyzed.As the most commonly used genetic markers in high-throughput sequencing and biological big data analysis,SNP markers are helpful for the identification of actual disease resistance of NBS-LRR genes in Solanum tuberosum varieties.