竹叶花椒黄酮抗氧化性及协同效应研究

Study on antioxidant activity and synergistic effect of flavonoids from Zanthoxylum armatum DC

目的:研究竹叶花椒黄酮提取物的抗氧化性以及协同效应。方法:采用DPPH自由基、ABTS自由基清除法评价竹叶花椒黄酮提取物的抗氧化活性,并通过加和法研究竹叶花椒黄酮提取物与维生素C和芦丁复配物的协同作用。结果:竹叶花椒黄酮提取物对DPPH自由基和ABTS自由基的半抑制浓度分别为0.03,0.062 mg/mL,具有较强的抗氧化活性。竹叶花椒黄酮提取物与维生素C或芦丁组成的复配物抗氧化活性高,且在复配比例为1∶1时抗氧化性最强,维生素C+黄酮提取物组成的复配物的抗氧化活性较芦丁+黄酮提取物组成的复配物高。结论:竹叶花椒黄酮具有强抗氧化性,其与维生素C及芦丁组成的复配物可提高对DPPH自由基和ABTS自由基的清除能力,具有抗氧化协同作用,可作为天然抗氧化剂的潜在来源。

Objective:This study aimed to investigate the antioxidation and synergistic effect of flavonoid extract of Zanthoxylum armatum DC.Methods:DPPH radical and ABTS radical scavenging methods were used to evaluate the antioxidant activity of flavonoids extract from Z.armatum,and the synergies of flavonoids extract with vitamin C and rutin complex were studied by adding method.Results:The semi-inhibitory concentrations of DPPH free radical and ABTS free radical IC 50 were 0.03 and 0.062 mg/mL,respectively,showing strong antioxidant activity.The antioxidant activity of the complex composed of flavonoid extract and vitamin C or rutin was high,and the antioxidant activity of the complex composed of vitamin C and flavonoid extract was higher than that of rutin and flavonoid extract when the ratio was 1∶1.Conclusion:The flavonoids of Z.armatum have strong antioxidant activity,and their complex composed of vitamin C and rutin can improve the scavenging capacity of DPPH free radical and ABTS free radicals.The extracted flavonoids have antioxidant synergistic effect,and can be used as a potential source of natural antioxidants.