储存条件对离体牛牙釉质粘接剪切强度的影响

Effect of various storage methods on shear bond strength of enamel of bovine teeth

目的:比较不同储存条件下牛牙的釉质粘接剪切强度,探讨适合储存离体牛牙的条件。方法:选择新鲜拔除的牛下前牙130颗,随机分为13组,每组10颗;其中,1组为对照组,12组为实验组。参照组在牛牙获取当天开始进行实验。实验组储存条件如下:4%甲醛溶液(4℃、30天,23℃、30天,4℃、90天,23℃、90天)、1%氯胺-T溶液(4℃、30天,23℃、30天,4℃、90天,23℃、90天)、蒸馏水(4℃、30天,23℃、30天,4℃、90天,23℃、90天)。储存完成后,将树脂粘接于牛牙釉质表面,测定其粘接剪切强度。采用SPSS 20.0软件包对测试结果进行统计学分析。结果:在23℃储存条件下,4%甲醛溶液和1%氯胺-T溶液储存30天和90天,粘接强度与新鲜牙釉质无显著差异。在4℃储存条件下,蒸馏水溶液储存30天和90天,粘接强度与新鲜牙釉质无显著差异。在4℃储存条件下,4%甲醛溶液和1%氯胺-T溶液储存30天,釉质粘接强度高于新鲜牛牙;90天时,与新鲜牛牙釉质粘接强度无显著差异。在23℃储存条件下,蒸馏水储存30天时粘接强度与新鲜牛牙釉质无显著差异;但到90天时,釉质粘接强度低于新鲜牛牙。结论:用4%甲醛溶液、1%氯胺-T溶液在23℃储存和蒸馏水4℃储存,粘接强度与新鲜牙釉质无显著差异,不随时间变化而变化。建议使用4%甲醛溶液和1%氯胺-T溶液在23℃、蒸馏水在4℃储存离体牛牙。

PURPOSE:The purpose of this study was to evaluate the effect of various storage methods on shear bond strength of enamel of bovine teeth and find the storage condition that could preserve the similar bond strength as the freshly extracted teeth.METHODS:One hundred and thirty freshly extracted bovine teeth were divided into 13 groups.One was the reference group and 12 were the experimental group.Each group contained 10 teeth.Teeth in the reference group were operated on the same day as the teeth were extracted,while teeth in the experimental groups were stored in different methods(4%formaldehyde solution at 4℃,23℃,1%chloramine T at 4℃,23℃,distilled water at 4℃,23℃).After stored for 30 days and 90 days,the bovine teeth were taken out and then the shear bond strength was tested.The data were analyzed with SPSS 20.0 software package.RESULTS:The bovine teeth stored in 4%formaldehyde and 1%chloramine T at 23℃and in distilled water at 4℃achieved similar bond strength as freshly extracted teeth at 30 days and 90 days,and the bond strength did not change over time.The bovine teeth stored in 4%formaldehyde solution and 1%chloramine T at 4℃at 30 days had higher shear bond strength than freshly extracted bovine teeth,but over time the bond strength reduced and reached the similar level at 90 days.The bovine teeth stored in distilled water at 23℃obtained similar bond strength as freshly extracted teeth at 30 days but over time the bond strength reduced until 90 days.CONCLUSIONS:Bovine teeth stored in 4%formaldehyde solution and 1%chloramine T at 23℃and in distilled water at 4℃achieved similar bond strength as freshly extracted teeth and does not change over time.These three methods are recommended for storing bovine teeth.