基于表面增强拉曼光谱技术的环境水样中杀鼠醚的现场快速检测方法

Rapid on-site detection of coumatetralyl in environmental water based on surface-enhanced Raman spectroscopy

杀鼠醚在水环境中的蓄积可对生态系统造成破坏,并对人体健康产生不利影响.本文利用表面增强拉曼光谱(SERS)技术,构建环境水中杀鼠醚的简单、快速定性及定量检测分析方法.采用化学合成法制备了粒径为45—60 nm的纳米金作为SERS活性基底,以便携式拉曼光谱仪作为检测平台,实现了3 min内完成环境水中杀鼠醚的检测.通过对实验条件进行优化,该方法的检出限低至1.53 ng·mL^(−1),加标回收率为90.2%—98.2%.在浓度为0.025—5μg·mL^(−1)范围内,杀鼠醚浓度与SERS信号强度间呈现出良好的线性关系,R2=0.990.与传统方法相比,该方法操作简单、快速、成本低廉,为水中杀鼠醚的现场检测分析提供了可靠的新选择.

The accumulation of coumatetralyl(CMTT)in the aquatic environment has caused damage to the ecosystem and adversely affected human health.In this paper,a simple and rapid qualitative and quantitative analysis of CMTT in environmental water was carried out using surfaceenhanced Raman spectroscopy(SERS).Gold nanoparticles with a particle size of 45—60 nm were prepared by chemical synthesis method and used as SERS substrate.Combined with a portable Raman spectrometer,the whole detection process could be completed within 3 minutes.By optimizing the experimental conditions,the detection limit of this method was 1.53 ng·mL^(−1),and the recovery was 90.2%—98.2%.In the range of 0.025—5μg·mL^(−1),the response between the concentration of CMTT and SERS signal intensity showed a clear linear dependence,R2=0.990.Compared with the traditional method,this method was simple,rapid and low-cost,providing a reliable application scenario for detecting and analyzing CMTT in water.