尿中甲基汞测定的直接测汞仪法

Determination of methylmercury in urine by direct mercury analyzer

目的建立尿中甲基汞的溶剂提取-直接测汞仪测定方法。方法尿样水解后,甲基汞经甲苯萃取,再由L-半胱氨酸溶液反萃取出来,直接加入到直接测汞仪中测定。结果尿中甲基汞浓度在0.2~ 50.0 μg/L线性关系良好,相关系数为0.999 9。方法批内精密度相对标准偏差(RSD)为5.04%~ 6.64%,批间精密度RSD为5.65%~8.11%,平均回收率分别为85.4%~95.5%,检出限为0.048 2 μg/L,定量下限为0.160 7 μg/L。结论直接测汞仪测定尿中甲基汞的检出限低、灵敏度高、稳定性好、操作简便,可用于尿中甲基汞的测定。

Objective To develop asolvent extraction-direct mercury analyzer method for determination of methylmercury in urine.Methods After the urinehydrolyzesd by hydrobromic acid,methylmercury was extracted by tolueneand reverse-extracted from L-cysteine solution,it was then detectedbydirect mercuryanalyzer.Results The linear range was 0.2-50.0μg/L,and the related coefficient was 0.9999.The relative standard deviations(RSD)within the group were 5.04%-6.64%,and the RSD between the group were 5.65%-8.11%.The average recovery efficiencies were 85.4%-95.5%.The detection limitation was 0.0482μg/L and the quantification concentrations was 0.1607μg/L.Conclusion The method,which has low detection limit,high sensitivity,easy to operate,is stability for the determination of methylmercury in urine.