白芍总苷调控舌癌HSC3细胞增殖、迁移和侵袭的机制研究

Mechanism of Total Glucosides of Paeony on the Proliferation,Migration and Invasion of Tongue Cancer HSC3 Cells

探讨白芍总苷(TGP)对舌癌HSC3细胞增殖、迁移和侵袭的影响及作用机制。TGP作用HSC3细胞或抑制LINC00319表达后,MTT检测HSC3细胞增殖,Transwell检测HSC3细胞的迁移和侵袭,蛋白印迹(Western Blot)检测细胞中CyclinD1、p21、MMP-2和MMP-9蛋白水平,qRT-PCR检测细胞中LINC00319和miR-608水平,双荧光素酶报告基因实验验证LINC00319和miR-608调控关系。TGP或抑制LINC00319表达后,HSC3细胞抑制率、p21蛋白及miR-608水平升高(P<0.05),细胞迁移和侵袭数、CyclinD1、MMP-2和MMP-9蛋白水平及LINC00319水平降低(P<0.05)。LINC00319靶向负调控miR-608表达,LINC00319过表达逆转TGP对HSC3细胞增殖、迁移和侵袭的影响(P<0.05)。TGP可能通过调控LINC00319/miR-608轴抑制舌癌HSC3细胞增殖、迁移和侵袭。

To investigate the effects of TGP on proliferation,migration and invasion of tongue cancer HSC3 cells and mechanism,after TGP treated HSC3 cells or inhibiting the expression of LINC00319,MTT detected the proliferation of HSC3 cells,Transwell detected the migration and invasion of HSC3 cells,Western blot detected the levels of CyclinD1,p21,MMP-2 and MMP-9 protein,and qRT-PCR detected the levels of LINC00319 and miR-608 in cells.The dual luciferase reporter assay validated the regulatory relationship between LINC00319 and miR-608.After TGP treatment,HSC3 cell inhibition rate,the level of p21 protein and miR-608 were increased(P<0.05),cell migration and invasion number,the level of cyclinD1,MMP-2,MMP-9 protein,and the level of LINC00319 were decreased(P<0.05).After inhibition of LINC00319 expression,HSC3 cell inhibition rate,the level of p21 protein and miR-608 were increased(P<0.05),cell migration and invasion number,the level of cyclinD1,MMP-2,MMP-9 protein were decreased(P<0.05).LINC00319 negatively regulated the expression of miR-608.The overexpression of LINC00319 could weaken the effect of TGP on the proliferation,migration and invasion of HSC3 cells(P<0.05).TGP may inhibit the proliferation,migration and invasion of tongue cancer HSC3 cells by regulating LINC00319/miR-608 axis.