畜禽肉中多类兽药残留的快速检测

Rapid Detection of Multiple Types of Veterinary Drug Residues in Livestock and Poultry Meat

建立了猪肉、牛肉、鸡肉中磺胺类、四环素类、喹诺酮类共15种兽药残留量的快速检测方法。样品经过Na_(2)EDTA-Mcllvaine缓冲液-2.5%乙酸乙腈溶液提取,经氯化钠、无水硫酸钠除水盐析后取乙腈相用高含碳量的C_(18)吸附粉吸附净化,再用0.1%甲酸水乙腈(75+25)定容后上机检测。结果显示,本方法测定三类混合药物的回收率在63.9%~104.0%,RSD在1.1%~12.2%,检测快速、灵敏度高、重复性好,定性定量准确可靠,适用于猪肉、牛肉及鸡肉中磺胺类、四环素类、喹诺酮类药物残留的快速检测。

A rapid method was established for the determination of 15 veterinary drug residues of sulfonamides,tetracyclines and quinolones in pork,beef and chicken.The samples were extracted by Na_(2)EDTA-Mcllvaine buffer-2.5%acetic acid acetonitrile solution,and the acetonitrile phase was purified by adsorption with high carbon content C_(18) adsorbent powder after dehydration salting by sodium chloride and anhydrous sodium sulfate,and then fixed with 0.1%formic acid aqueous acetonitrile(75+25)and detected on the machine.The results showed that the method determines the recovery of the three types of mixed were 63.9%~104.0%,RSD were 1.1%~12.2%,rapid detection,high sensitivity,good repeatability,accurate and reliable qualitative and quantitative,suitable for the rapid detection of sulfonamides,tetracyclines,quinolones drug residues in pork,beef and chicken.