基于^(1)H NMR代谢组学技术的黄刺玫果实抗衰老作用研究

Research on the Anti-aging Effect of Rosa xanthina Lindl Fruits Based on ^(1)H NMR Metabolomics

目的:采用^(1)H NMR代谢组学方法,探讨黄刺玫果实对D-半乳糖致衰老模型大鼠的干预作用。方法:皮下注射D-半乳糖(400 mg/kg)致大鼠亚急性衰老,考察黄刺玫果实提取物(1.6、3.2、6.4 g/kg)的抗衰老作用。造模30d后采集大鼠血清,检测血清生化指标SOD(superoxide dismutase)、 LPO(lipid peroxide)及Hyp(hydroxyproline)水平,并进行^(1)H NMR代谢组学检测,结合多元统计分析研究黄刺玫果实提取物高、中、低剂量的抗衰老作用。结果:与模型组相比,黄刺玫果实提取物可显著升高血清SOD(P<0.01)、Hyp(P<0.01)水平,降低LPO(P<0.05,P<0.01)水平。与对照组相比,模型组大鼠血清中13种差异代谢物发生显著(P<0.05,P<0.01)性变化。给予黄刺玫后,高剂量组可显著(P<0.05,P<0.01)回调脂质、精氨酸、甘油酯、氧化三甲胺、甘氨酸、丙氨酸及N-乙酰糖蛋白7种代谢物,中剂量组可显著调节氧化三甲胺、N-乙酰糖蛋白、亮氨酸3种代谢物,而低剂量组仅显著回调磷脂酰胆碱。三组药效指数(EI)由大到小分别为高剂量组、中剂量组、低剂量组,表明高剂量组的抗衰老效果最佳。结论:黄刺玫果实可能通过调节氧化应激、促进胶原蛋白合成,以及调节氨基酸代谢、肠道菌群代谢等途径延缓衰老。

Objective:The anti-aging effect of the fruits of Rosa xanthina Lindl(RF)was investigated by the ^(1)H NMR based metabolomics using D-galactose-induced aging rats.Methods:Rat subacute senile model was established by subcutaneous injection of D-galactose(400 mg/kg),and the anti-aging effects of RF were studied at three different doses(1.6,3.2,6.4 g/kg).On the 30th day,rat serum was collected and the serum superoxide dismutase(SOD),lipid peroxide(LPO)and hydroxyproline(Hyp)were determined.The anti-aging effects of high,middle,low dose of RF were investigated by 1H NMR metabolomics,and combined with multivariate statistical analysis.Results:When compared with the model group,RF extracts could increase serum SOD and Hyp levels(P<0.01)and decrease LPO level(P<0.05,P<0.01)significantly.For the rats in the model group,13 differential metabolites in serum were changed(P<0.05,P<0.01)significantly.After treatment by RF,seven metabolites including lipid,arginine,glycerol,trimethylamine N-Oxide(TMAO),glycine,alanine and N-acetyl glycoprotein were regulated in the high dose group,and then three metabolites including TMAO,N-acetyl glycoprotein and leucine were altered in the middle dose group,while only phosphatidylcholine(PC)was decreased in the low dose group(P<0.05,P<0.01)significantly.The best ranking of Efficacy index(EI)in three groups was high,middle and low,indicating that the high dose of RF showed the best anti-aging effect.Conclusion:RF exhibited the anti-aging effect probably through regulating oxidative stress,promoting collagen synthesis,and also related with various metabolic pathways,such as amino acid metabolism and gut microbiota metabolism.