阿胶制品中典型明胶特征性多肽的鉴定

Identification of Characteristic Peptides from Typical Gelatin in Ejiao Products

为了研究不同类型阿胶制品中典型明胶的来源,本文选取9种市面上常见的阿胶制品(阿胶糕、阿胶口服液和阿胶颗粒各3种)为研究对象,分别添加质量分数为1%的牛明胶和猪明胶,采用高效液相色谱-高分辨率质谱联用技术(high-performance liquid chromatography-mass spectrometry,HPLC-MS/MS)鉴定这9种阿胶制品中典型明胶的特征性多肽。结果表明,不同数量的典型明胶特征性多肽被检出于9种阿胶制品。进一步分析发现,所有阿胶制品共有的阿胶、牛明胶、猪明胶的特征性多肽分别为25、3、14条,其中,未经修饰的特征性多肽依次为7、2、3条。在上述共有特征性多肽中,源自α_(1)链的分别有22、2、5条,源自α_(2)链的分别为3、1、9条。这些共有特征性多肽可作为HPLC-MS/MS鉴定复杂体系中明胶源的依据,对不同阿胶制品中明胶来源的鉴定具有指导意义。

In order to study the source of typical gelatin in different types of Ejiao products,nine common Ejiao products on the market including three kinds of Ejiao cake,Ejiao oral liquid and Ejiao granule,were selected as the research objects.Moreover,1%bovine gelatin and porcine gelatin were added in Ejiao products,respectively.High-performance liquid chromatography-mass spectrometry(HPLC-MS/MS)was used to identify the characteristic peptides of typical gelatin including donkey gelatin,bovine gelatin,and porcine gelatin.The results showed that different characteristic peptides of typical gelatin were detected in nine kinds of Ejiao products.Through further analysis,it was found that the common characteristic peptides of donkey gelatin,bovine gelatin,and porcine gelatin shared by these 9 Ejiao products were 25,3,and 14,respectively,where the unmodified characteristic peptides were 7,2,and 3 in turn.Among them,22,2,and 5 common characteristic peptides were derived from the α_(1) chain,respectively.Meanwhile,3,1,and 9 common characteristic peptides were derived from the α_(2) chain,respectively.These common characteristic peptides could be used as the basis for the identification of gelatin sources in complex systems by HPLC-MS/MS,and give guiding significance in gelatin identification for different Ejiao products.