摘要
为探究常温酸奶发酵剂发酵过程中分泌的胞外多糖(EPS)对小鼠结肠炎的缓解作用,本研究在常温酸奶发酵剂A和发酵剂B分别发酵后的常温酸奶中提取纯化胞外多糖,测定其分子特性、结构形貌和单糖组成,同时通过葡聚糖硫酸钠(DSS)诱导小鼠结肠炎,分析了两种胞外多糖对小鼠体重、结肠组织病理学、小鼠炎症因子、髓过氧化物酶(MPO)和紧密连接蛋白(ZO-1、Occludin)表达量的影响。结果表明,EPS-A和EPS-B在分子形貌和单糖组成上均有明显差异。EPS-A结构略疏松,由盐酸氨基半乳糖、半乳糖和葡萄糖3种单糖构成,其摩尔比为0.345:0.21:0.435;EPS-B结构致密,由盐酸氨基半乳糖、盐酸氨基葡萄糖、半乳糖和葡萄糖4种单糖构成,其摩尔比为0.421:0.05:0.207:0.322。各胞外多糖治疗组均能够恢复炎症性肠病(IBD)小鼠体重,但是均不能显著降低疾病活动指数(DAI)评分;EPS-A高剂量组结肠形态接近空白对照组,两者的结肠长度和结肠系数没有显著性差异(P>0.05);结合不同治疗组中炎症因子TNF-α、IL-1β、IL-10表达量,EPS-A高剂量组对炎症因子的反应效果最好,和药物治疗组的反应效果无显著性差异(P>0.05);EPS-A胞外多糖高剂量组和低剂量组同药物治疗组的ZO-1和Occludin蛋白的表达量显著性提高(P<0.05),EPS-B高剂量组ZO-1蛋白的表达量相较于模型组也有显著性提高(P<0.05),但是效果不如EPS-A组;不同胞外多糖治疗组在降低MPO活性方面均没有显著改善(P>0.05)。综合以上结果可知EPS-A能够显著改善小鼠肠炎情况,其效果优于EPS-B。
In order to study the relieving effect of extracellular polysaccharides(EPS-A,EPS-B)which were extracted and purified from long shelf-life yogurt fermented by long shelf-life yogurt culture A and culture B,their molecular characteristics,structural morphology and monosaccharide composition were determined,and the mouse weight,influence of colon histopathology,the expression of inflammatory factors,the content of myeloperoxidase(MPO)and tight junction proteins(ZO-1,Occludin)in mice were also analyzed through constructing an animal model of colonic inflammation in mice by dextran sulfate sodium(DSS).The results showed that there were significantly differences on the molecular morphology and monosaccharide compositions between EPS-A and EPS-B.The EPS-A was slightly loose in structure,composed of three monosaccharides(galactosamine hydrochloride,galactose and glucose)with a molar ratio of 0.345:0.21:0.435.The structure of EPS-B was compact and it composed of four monosaccharides(galactosamine hydrochloride,glucosamine hydrochloride,galactose and glucose)with a molar ratio of 0.421:0.05:0.207:0.322.The weight of IBD mice was recovered in all the exopolysaccharide treatment groups,but there was no significant decrease in the disease activity index(DAI)score.The colon morphology of the high dose group of EPS-A was close to that of the blank control group,and there was no significant difference in colon length and colon coefficient between the two groups(P>0.05),the same below).Combined with the expression of inflammatory factor(TNF-α,IL-1β,IL-10)in different treatment groups,the high dose group of EPS-A had the best response to those inflammatory factors,and there was no significant difference between it and the drug treatment group(P>0.05).The expression of ZO-1 and occludin protein in the high-dose and low-dose groups of EPS-A was significantly improved and same as the drug treatment group(P<0.05).The expression of ZO-1 protein in the high-dose group of EPS-B was also significantly higher than that in the model group,but the effect was not as good as that in the EPS-A group(P<0.05).There was no significant improvement in reducing MPO activity in different extracellular polysaccharide treatment groups compared with the model group(P>0.05).In summary,it could be seen that EPS-A can significantly improve the enteritis of mice,and its effect was better than that of EPS-B.
作者
户行宇
姚梦柯
孙婷
马霞
孙倩
洪维鍊
杨贞耐
HU Hangyu;YAO Mengke;SUN Ting;MA Xia;SUN Qian;HONG Weilian;YANG Zhennai(Beijing Technology and Business University,School of Food and Health,Beijing 100048,China;Inner Mongolia Yili Industrial Group Co.,Ltd.,Huhehot 010080,China)
出处
《食品工业科技》
CAS
北大核心
2023年第12期378-387,共10页
Science and Technology of Food Industry
基金
呼和浩特市科技计划项目(2020-合-2)。