RP-HPLC法同时测定紫苏叶提取物没食子酸、迷迭香酸、木犀草素含量研究

Simultaneous Determination of Gallic Acid,Rosmarinic Acid and Luteolin in Perilla Leaves Extract by RP-HPLC

目的建立安徽地区紫苏叶中没食子酸、迷迭香酸和木犀草素成分的含量同时检测方法。方法采用反相高效液相色谱法,Symmetry C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇(A)-0.1%磷酸溶液(B)为流动相梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长272 nm同时检测没食子酸、迷迭香酸和木犀草素含量。采用SPSS 17.0对测定结果进行分析,比较本地主产区紫苏叶中各成分的含量差异。结果没食子酸、迷迭香酸和木犀草素分别在33.80~101.2μg/mL,33.60~100.8μg/mL,34.00~102.0μg/mL呈线性关系,平均加样回收率分别为98.04%(RSD=1.50%,n=6),96.40%(RSD=0.53%,n=6)和96.49%(RSD=1.89%,n=6)。结论此方法简单方便,稳定可重复,可作为安徽地区主产地紫苏叶没食子酸、迷迭香酸和木犀草素成分含量的检测依据。

Objective To establish a method for the simultaneous detection of gallic acid,rosmarinic acid and luteolin in perilla leaves in Anhui area.Method The content of gallic acid,rosmarinic acid and lignocaine was simultaneously determined by reversed-phase high performance liquid chromatography(RP-HPLC)on a Symmetry C18(250 mm×4.6 mm,5μm)column with methanol(A)-0.1%phosphoric acid solution(B)as the mobile phase in a gradient elution at a flow rate of 1.0 mL/min and a column temperature of 30℃with the detection wavelength of 272 nm.SPSS 17.0 was used to analyze the determination results,and the differences in the contents of various components in perilla leaves from the main local producing areas were compared.Results The gallic acid,rosmarinic acid and luteolin showed linear relationship at 33.80~101.2μg/mL,33.6~100.8μg/mL,34.00~102.0μg/mL,respectively,and the average recovery rate was 98.04(RSD=1.50%,n=6),96.40(RSD=0.53%,n=6)and 96.49(RSD=1.89%,n=6).Conclusion This method is simple,convenient,stable and repeatable,and can be used as the simultaneous detection of gallic acid,rosmarinic acid and luteolin in the main producing area of Perilla frutescens in Anhui.