环状RNA hsa_circZDHHC21_004对60Coγ射线照射后人小肠上皮细胞HIEC-6增殖能力的影响

Effect of circular RNA hsa_circZDHHC21_004 on the proliferation of the human small intestinal epithelial cells HIEC-6 exposed to 60Coγ-rays

目的探讨环状RNA hsa_circZDHHC21_004对电离辐射照射后人小肠上皮细胞HIEC-6增殖能力的影响。方法60Coγ射线照射人小肠上皮细胞HIEC-6,吸收剂量为0、5、10和15 Gy,剂量率为1 Gy/min。检测HIEC-6中hsa_circZDHHC21_004的表达水平随照射剂量的变化趋势。构建hsa_circZDHHC21_004稳定敲低细胞系,通过CCK-8实验和克隆形成实验,检测hsa_circZDHHC21_004表达水平的变化对辐射照射后HIEC-6增殖能力的影响。结果HIEC-6中hsa_circZDHHC21_004表达水平随辐射剂量的增高而增高(1.00±0.24、1.34±0.28、1.85±0.31、2.80±0.64,F=10.86,P=0.008)。10和15 Gy照射后,hsa_circZDHHC21_004的表达水平与0 Gy之间的差异具有统计学意义(P<0.05)。CCK-8结果显示,hsa_circZDHHC21_004稳定敲低细胞的增殖在照射10 Gy后24、48、72 h均高于对照细胞的增殖,且差异具有统计学意义(t=-6.25、-5.83、-7.75,P<0.001)。Hsa_circZDHHC21_004稳定敲低细胞经2和5 Gy照射后,其克隆形成率均高于对照细胞的克隆形成率,且差异均有统计学意义(t=-7.45、-8.83,P<0.01)。结论电离辐射可上调人小肠上皮细胞HIEC-6中hsa_circZDHHC21_004表达水平。Hsa_circZDHHC21_004影响了电离辐射照射后HIEC-6的增殖。

Objective To study the influence of circular RNA hsa_circZDHHC21_004 on the proliferation of human small intestinal epithelial cells HIEC-6 after 60Coγ-rays exposure.Methods HIEC-6 cells were exposed to 60Coγ-rays at 0,5,10,and 15 Gy with a dose rate of 1 Gy/min.The expression level of hsa_circZDHHC21_004 in the irradiated HIEC-6 cell was detected.Hsa_circZDHHC21_004 was knocked-down to investigate the influences of hsa_circZDHHC21_004 on the proliferation of irradiated HIEC-6 cells by CCK-8 assay and colony formation assay.Results The expression level of hsa_circZDHHC21_004 in HIEC-6 cells was upregulated by(1.00±0.24),(1.34±0.28),(1.85±0.31),and(2.80±0.64)times of control after 0,5,10,and 15 Gy irradiation,respectively and there were significant difference between 10 or 15 Gy group and 0 Gy group(F=10.86,P=0.008).Knockdown of hsa_circZDHHC21_004 significantly increased the proliferation rate of HIEC-6 cells at 24,48,and 72 h after 10 Gy irradiation compared with non-irradiated control(t=-6.25,-5.83,-7.75,P<0.001).Under 2 and 5 Gy irradiation,the clone formation rates of the hsa_circZDHHC21_004 knockdown cells were significantly higher than those of the control(t=-7.45,-8.83,P<0.01).Conclusions Hsa_circZDHHC21_004 is increased after irradiation and influenced the proliferation of irradiated HIEC-6 cells.