pN0期非小细胞肺癌VEGF、Ki-67、p53表达与淋巴结微转移的相关性

Study on the correlation between the expression levels of VEGF,Ki-67,p53 and lymph node micrometastasis in pN0 stage non-small cell lung cancer

目的:探讨pN0期非小细胞肺癌血管内皮生长因子(VEGF)、Ki-67、p53表达与淋巴结微转移相关性。方法:选择术后经常规病理检查证实为pN0期的非小细胞肺癌病人93例及手术治疗的非肺癌病人45例,采用免疫组织化学法检测肺癌组织及正常肺组织中VEGF、Ki-67和p53表达情况,分析肺癌组织中VEGF、Ki-67、p53表达与病人临床病理学特征的关系,并采用ROC曲线分析其对淋巴结微转移的诊断价值。结果:肺癌组织中VEGF、Ki-67和p53表达阳性率均明显高于正常肺组织(P<0.01)。不同TNM分期的非小细胞肺癌病人肺组织VEGF、Ki-67、p53表达阳性率差异均有统计学意义(P<0.05),而不同性别、年龄、病理类型、肿瘤最大径及吸烟史病人的VEGF、Ki-67、p53表达阳性率差异均无统计学意义(P>0.05)。淋巴结微转移的非小细胞肺癌病人VEGF、Ki-67、p53表达评分均明显高于无淋巴微转移病人(P<0.01)。VEGF、Ki-67、p53评分对非小细胞肺癌病人淋巴结微转移诊断AUC分别为0.816、0.877、0.821。结论:pN0期非小细胞肺癌病人肺组织VEGF、Ki-67及p53表达与肺癌的发生发展有关,且与病人淋巴结微转移有关,可作为病人淋巴结微转移的潜在检测指标。

Objective:To investigate the correlation between the expression levels of vascular endothelial growth factor(VEGF),Ki-67,p53 and lymph node micrometastasis in pN0 stage non-small cell lung cancer(NSCLC).Methods:Ninety-three NSCLC patients confirmed by routine pathological examination and 45 non-lung cancer patients treated with operation were selected.The expression levels of VEGF,Ki-67 and p53 in lung cancer tissue and normal lung tissue were detected by immunohistochemistry,the relationship between the expression levels of VEGF,Ki-67 and p53 in lung cancer and clinicopathological characteristics of patients were analyzed,and the ROC curve was used to analyze its diagnostic value for lymph node micrometastasis.Results:The positive rates of VEGF,Ki-67 and p53 expression in lung cancer tissues were significantly higher than those in normal lung tissues(P<0.01).The differences of the positive rates of VEGF,Ki-67 and p53 expression in lung tissues of NSCLC patients with different TNM stages were statistically significant(P<0.05),but which was not related to the gender,age,pathological type,tumor size and smoking history(P>0.05).The scores of VEGF,Ki-67 and p53 expression in lung tissue of patients with lymph node micrometastasis were significantly higher than those of patients without lymph node micrometastasis(P<0.01).The AUC values of the scores of VEGF,KI-67 and p53 in the diagnosis of lymph node micrometastasis in NSCLC patients were 0.816,0.877 and 0.821,respectively.Conclusions:The expression levels of VEGF,Ki-67 and p53 in lung tissue of patients with pN0 stage NSCLC is closely related to the occurrence and development of lung cancer,and lymph node micrometastasis.It can be used as a potential detection index of lymph node micrometastasis.