碱提酸沉法提取补骨脂中补骨脂乙素的工艺研究

Study on the extraction process of isobavachalcone from Psoraleae corylifolia using alkaline extraction and acid precipitation

目的:通过碱提酸沉的方法对补骨脂乙素的提取进行工艺优化。方法:以补骨脂乙素的含量为指标,通过单因素试验和正交试验考察料液比、碱液浓度、碱提时间、酸沉pH、酸沉时间以及超声时间对补骨脂乙素提取工艺的影响。结果:碱提酸沉法提取补骨脂乙素的最佳工艺为:料液比为1∶10、碱液浓度为4%、碱提时间为2 h、酸沉pH为6、酸沉时间为0.5 h、超声时间为20 min。结论:本研究优化的最佳工艺条件具有良好的复重性,且稳定可靠、经济环保,适合大量提取补骨脂中的补骨脂乙素。

Objective:To optimize the extraction process of isobavachalcone by alkaline extraction and acid precipitation.Methods:The content of isobavachalcone was set as the objective parameter.The effects of solid-liquid ratio,alkali concentration,alkali extraction time,acid precipitation pH,acid precipitation time and ultrasound time on extraction process of isobavachalcone were investigated through single factor test and orthogonal test.Results:The optimal extraction conditions of isobavachalcone by alkaline extraction and acid precipitation were as follows:the ratio of solid-liquid for 1∶10,alkali concentration for 4%,alkali extraction time for 2 h,acid precipitation pH 6,acid precipitation time for 0.5 h and ultrasound time for 20 min.Conclusions:In this study,the optimal process conditions have the good repeatability,and it is stable,reliable,economical and environmental-friendly,which can greatly improve the extraction rate of isobavachalcone from Psoralea corylifolia.