肝细胞癌中lncRNA SNHG25的高表达与不良预后相关

High expression of lncRNA SNHG25 in hepatocellular carcinoma is associated with poor prognosis

目的:评价lncRNA SNHG25在肝细胞癌中的表达及其临床意义、预后价值以及潜在有相互作用的分子。方法:通过用qRT-PCR方法检测肝细胞癌细胞系和正常人肝细胞系中lncRNA SNHG25的表达。其次通过UALCAN和仙桃学术,并使用TCGA数据库分析lncRNA SNHG25在肝癌组织和正常肝脏组织中的表达水平、临床应用意义以及临床病理因素与lncRNA SNHG25表达的相关性。然后使用GEPIA来分析lncRNA SNHG25表达与患者预后之间的关系。最后使用Starbase 3.0预测了可能与lncRNA SNHG25相互作用的分子。结果:通过qRT-PCR方法检测,在大部分肝细胞癌细胞系中lncRNA SNHG25的表达量高于正常肝细胞系。与正常组织相比较,肝细胞癌组织中lncRNA SNHG25的表达显著升高;lncRNA SNHG25的表达与组织分级、甲胎蛋白(AFP)水平、种族相关,但与年龄、性别无明显相关。GEPIA结果表明lncRNA SNHG25的高表达与无病生存期显著相关,而与总生存期无明显相关。Starbase 3.0分析得到GNPTAB、C19orf25、METTL16、ELF4、AC139365.1以及18个miRNA可能与lncRNA SNHG25相互作用的分子。结论:lncRNA SNHG25在肝细胞癌中明显高表达,并且与不良预后相关,其有可能成为一种有效的临床生物标志物和肝细胞癌的治疗靶点。

Objective:To evaluate the expression of lncRNA SNHG25 in hepatocellular carcinoma and its clinical significance,prognostic value and potentially interacting molecules.Methods:The expression of lncRNA SNHG25 in hepatocellular carcinoma cell lines and normal human liver cell lines was detected by qRT-PCR method.Secondly,through UALCAN and Xiantao Academic,TCGA database was adopted to analyze the expression level of lncRNA SNHG25 in liver cancer tissues and normal liver tissues,clinical application significance and the correlation between clinicopathological factors and the expression of lncRNA SNHG25.Then the GEPIA was used to analyze the relationship between lncRNA SNHG25 expression and the patients'prognosis.Finally,Starbase 3.0 was used to predict the molecules that may interact with lncRNA SNHG25.Results:Detected by qRT-PCR method,the expression of lncRNA SNHG25 in most of hepatocellular carcinoma cell lines were higher than in normal hepatic cell lines.Compared with that in normal tissues,the expression of lncRNA SNHG25 in liver cancer tissues was significantly increased,and the expression of lncRNA SNHG25 was related to tissue grade,alpha-fetoprotein(AFP)level,and race,but not related to age or gender.GEPIA results showed that the high expression of lncRNA SNHG25 was significantly related to disease-free survival,but not to overall survival.The Starbase 3.0 analysis revealed that GNPTAB,C19orf25,METTL16,ELF4,AC139365.1,and 18 miRNAs molecules might interact with lncRNA SN-HG25.Conclusion:The results of our analysis show that lncRNA SNHG25 is significantly and highly expressed in hepatocellular carcinoma and is associated with a poor prognosis.It may serve as an effective clinical biomarker and a therapeutic target for hepatocellular carcinoma patients.