心肌梗死介入治疗后sTRAIL-R2表达与颈动脉斑块细胞凋亡及炎症反应的相关性

Correlation between sTRAIL-R2 expression and carotid plaque apoptosis and inflammatory response in patients with myocardial infarction after interventional therapy

目的探究心肌梗死患者介入治疗后可溶性肿瘤坏死因子相关凋亡诱导配体受体2(sTRAIL-R2)表达与颈动脉斑块细胞凋亡及炎症反应的相关性。方法选择2021年1月至2022年5月该院收治的心肌梗死行介入治疗后患者102例作为研究对象,对其行颈动脉内膜切除术获取颈动脉斑块片段,根据sTRAIL-R2表达水平分为sTRAIL-R2高表达组和sTRAIL-R2低表达组。检测患者斑块组织Bax、半胱天冬氨酸蛋白酶(Caspase)-8、Caspase-3活性,CD45、CD68表达水平及白细胞介素(IL)-6、IL-10、C反应蛋白(CRP)、肿瘤坏死因子(TNF)-α和IL-1β水平,检测患者斑块组织细胞凋亡相关蛋白表达并分析sTRAIL-R2表达水平与患者斑块组织细胞凋亡、炎症反应的相关性。结果sTRAIL-R2高表达组患者斑块组织Caspase-8、Caspase-3活性,Bax、Caspase-3蛋白表达水平,CD45、CD68阳性细胞检出数,IL-6、IL-10、CRP、TNF-α、IL-1β水平均高于sTRAIL-R2低表达组,B淋巴细胞瘤-2基因(Bcl-2)蛋白表达水平低于sTRAIL-R2低表达组,差异均有统计学意义(P<0.05)。sTRAIL-R2表达水平与Caspase-8、Caspase-3活性,CD45、CD68、IL-6、IL-10、CRP、TNF-α、IL-1β水平与Bax、Caspase-3蛋白表达水平均呈正相关,与Bcl-2蛋白表达水平呈负相关(P<0.05)。结论sTRAIL-R2高表达可引起颈动脉粥样硬化斑块组织Caspase-8、Caspase-3活性升高,细胞凋亡相关蛋白表达水平上调,并引起斑块炎症反应加剧,可能导致易损斑块出现。

Objective To explore the correlation between expression of soluble tumor necrosis factor-related apoptosis-inducing ligand receptor 2(sTRAIL-R2)and apoptosis of carotid plaque cells and inflammatory response in patients with myocardial infarction after interventional therapy.Methods A total of 102 patients with myocardial infarction admitted to this hospital from January 2021 to May 2022 after interventional therapy were selected as research objects,and carotid plaque fragments were obtained by carotid endarterectomy.According to the expression level of sTRAIL-R2,sTRAIL-R2 high expression group and sTRAIL-R2 low expression group were divided.The activities of Bax,Caspase-8 and Caspase-3,the expression levels of CD45 and CD68,and the levels of interleukin(IL)-6,IL-10,C-reactive protein(CRP),tumor necrosis factor(TNF)-αand IL-1βin plaque tissue of patients were detected.The expression of apoptosis-related proteins in plaque tissue was detected and the correlation between the expression level of sTRAIL-R2 and apoptosis and inflammatory response in plaque tissue was analyzed.Results Caspase-8 and Caspase-3 activities,Bax and Caspase-3 protein expression levels,CD45 and CD68 positive cells and the levels of IL-6,IL-10,CRP,TNF-αand IL-1βin plaque tissue of patients with sTRAIL-R2 high expression were higher than those of sTRAIL-R2 low expression group,and the expression of Bcl-2 protein was lower than that of sTRAIL-R2 low expression group,and the differences were statistically significant(P<0.05).The expression level of sTRAIL-R2 was positively correlated with the activities of Caspase-8 and Caspase-3,the levels of CD45,CD68,IL-6,IL-10,CRP,TNF-αand IL-1β,and the protein expression levels of Bax and Caspase-3.The expression of Bcl-2 protein was negatively correlated with the expression of Bcl-2 protein(P<0.05).Conclusion The high expression of sTRAIL-R2 can increase the activities of Caspase-8 Caspase-3 and the expression of apoptosis related proteins,and could aggravate the inflammatory response of carotid plaqueatherosclerotic,which may lead to the appearance of vulnerable plaque.