实验性脊髓损伤后大脑血管可塑性的磁共振血管尺寸成像研究

In vivo MR Vessel Size Imaging of Brain Vascular Plasticity After Experimental Spinal Cord Injury

本文应用7 T高场磁共振血管尺寸成像技术研究了大鼠脊髓半切损伤后大脑血管的可塑性.通过感兴趣区域分析发现,损伤4周后,与损伤部位同侧锥体相比,对侧锥体区域的平均血管直径、微血管密度和血管尺寸指数显著增加,提示对侧皮质脊髓束白质区域血管生成或活化.该结果经由免疫荧光实验证实,损伤4周后,对侧锥体区域血小板内皮细胞粘附分子和胶质纤维酸性蛋白的染色强度也显著增加.以上结果表明磁共振血管尺寸成像技术可为脊髓损伤后大脑相关区域的血管生成提供有价值的信息,并有可能成为诊断脊髓损伤患者脑部血管病变的新工具.

In vivo magnetic resonance vessel size imaging at 7 T high field was used to explore the changes in cerebral vascular plasticity after spinal cord hemisection injury in rats.Region of interest(ROI)analysis showed that four weeks after injury,mean vessel diameter(mVD),microvascular density(Density)and vessel size index(VSI)values were significantly increased in the contralateral side compared with the ipsilateral pyramid of the injury site,suggesting angiogenesis or vascular activation in the white matter region of the contralateral corticospinal tract(CST).Immunofluorescence results showed that the intensity of staining for platelet endothelial cell adhesion molecule 1(CD31)and glial fibrillary acidic protein(GFAP)also increased significantly in the contralateral pyramid four weeks after injury.These results suggested that magnetic resonance vessel size imaging could provide valuable information on neovascularization in brain after spinal cord injury and may be a novel tool to diagnose brain vascular pathologies in spinal cord injury patients.