辣椒WOX转录因子家族的鉴定、进化与表达分析

Identification,Evolution and Expression Analysis of WOX Gene Family in Capsicum annuum

WUSCHEL(WUS)相关的同源异型盒(WOX)转录因子家族是一类植物特异性转录因子,维系植物干细胞动态平衡、茎尖分生组织形成、胚胎发育等重要生命活动。利用生物信息学方法,在遵辣1号辣椒中鉴定到10个WOX基因,命名为CaWUS—CaWOX13。CaWOXs不均等地分布在5条染色体上,被分为3个支系,亚细胞预测全部定位在细胞核上。不同支系间基因数目差异较大,同一支系的成员具有相似的基因结构和保守基序。CaWOX家族启动子包含14种有关植物生长发育、激素调控和逆境胁迫的作用元件,分布最广的为茉莉酸甲酯应答元件(MeJA responsiveness)。CaWOX家族内没有发现串联重复和大片段复制,但和拟南芥、番茄存在共线性关系。CaWUS作为关键蛋白,在蛋白质互作网络中承担枢纽作用。CaWOXs的基因表达量具有显著的差异性,部分基因在根、茎以及果实发育过程中具有重要的调控作用。

The WUSCHEL(WUS)-related homeobox(WOX)transcription factor family is a kind of plant specific transcription factors,which maintain the dynamic balance of plant stem cells,shoot tip meristem formation,embryonic development and other important life activities.In this study,10 WOX genes were identified in pepper(Capsicum annuum)variety Zunla-1 by using bioinformatics methods,named CaWUS to CaWOX13.CaWOXs were unevenly distributed on 5 chromosomes and are divided into 3 branches.All subcellular predictions showed being located in the nucleus.The number of genes varied greatly among different branches,and members of the same branch had similar gene structures and conserved motifs.CaWOX family promoters contained 14 kinds of action elements related to plant growth and development,hormone regulation and stress,and the most widely distributed was methyl jasmonate response element.No tandem duplication and large fragment duplication were found in CaWOX family,but there was a collinear relationship with Arabidopsis thaliana and Solanum lycopersicum.As a key protein,CaWUS played a pivotal role in the protein interaction network.The gene expression of CaWOXs was significantly different,and some genes played an important role in the regulation of root,stem and fruit development.