黄粉虫粪发酵物对陈年番茄种子萌发的影响

Effect of Yellow Mealworm Excrement Fermentation on Germination of Aged Tomato Seeds

为探究添加枯草芽孢杆菌、EM菌剂、虫生真菌、哈茨木霉NF9菌种和不加菌种的黄粉虫粪发酵物对陈年番茄种子萌发的影响,以中农长丰为试验材料,采用培养皿纸上发芽法,研究不同虫粪发酵物浸提液在不同体积分数下对番茄发芽率、发芽指数、活力指数、根长、整株鲜质量等指标的影响。结果表明,添加EM菌剂的虫粪发酵物浸提液促进番茄种子萌发作用最明显,番茄发芽势、发芽指数、整株鲜质量和胚根长度均在浸提液体积分数为4%时最大,分别达到50.00%、10.73、0.06 g和5.70 cm,比对照(蒸馏水处理)分别提高了233.33%、126.85%、90.32%和39.02%;番茄发芽率、活力指数和胚芽长度在浸提液体积分数为6%时达最大,分别为70.00%、73.64和7.00 cm。其中,番茄发芽势、发芽指数、活力指数、整株鲜质量、胚芽长度比其他虫粪浸提液处理的最大值分别增加141.90%、67.39%、135.42%、22.92%和20.07%。添加枯草芽孢杆菌、虫生真菌、哈茨木霉NF9菌种的虫粪发酵物浸提液和黄粉虫粪自然发酵物浸提液对番茄种子萌发也有促进作用。综上,不同虫粪发酵物浸提液在一定范围内均能促进番茄种子萌发,其中,添加EM菌剂的黄粉虫粪发酵物浸提液在体积分数为4%时促进番茄种子萌发作用最明显。

To investigate the effects of yellow mealworm excrement fermentation with bacillus subtilis,EM agent,entomogenous fungi,Trichoderma harziana NF9 strain,and without strain on aged tomato seed germination,in this study,taking Zhongnong Changfeng as the experimental material,the effects of different insect excrement fermentation extracts at different volume concentration on tomato germination rate,germination index,vigor index,root length,and whole plant fresh weight were studied by the method on petri dish paper.The results showed that the extract of the fermentation of insect excrement with EM agent had the most obvious function to promote the germination of tomato seeds.The germination potential,germination index,whole plant fresh weight,and radicle length of tomato were the highest at 4%,they were 50.00%,10.73,0.06 g,and 5.70 cm,respectively,which were 233.33%,126.85%,90.32%,and 39.02%higher than those of the control(distilled water treatment).The germination rate,vigor index,and germ length were the highest at 6%,they were 70.00%,73.64,and 7.00 cm,respectively.Among them,the germination potential,germination index,vigor index,whole plant fresh weight,and germ length of tomato were 141.90%,67.39%,135.42%,22.92%and 20.07%higher than those of other insect excrement extracts.The addition of bacillus subtilis,entomogenous fungi,T.harzianum NF9 strain of insect excrement fermentation extract and yellow mealworm manure natural fermentation extract also promoted tomato seed germination.Therefore,different extracts of insect excrement fermentation could promote the germination of tomato seeds in a certain range,in which the yellow mealworm excrement fermentation extract with EM agent at 4%of volume concentration possessed the most obvious function to promote tomato seed germination.