多糖的高级结构鉴定分析的研究进展

Research progress of identification and analysis methods for advanced structure of polysaccharides*

多糖是自然界中普遍存在的生物大分子,具有多种药理活性,多用于功能性食品和治疗剂。多糖的生物活性取决于其化学结构,本文介绍了二维核磁共振(2D NMR)、X-射线衍射法、毛细管电泳-质谱联用技术(CE-MS)、基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)、圆二色光谱(CD)、扫描电子显微镜(SEM)、原子力光谱(AFM)、差示扫描量热法(DSC)以及分子动力学模拟技术等多糖分析鉴定方法,并对今后的研究工作进行了展望。

Polysaccharide is a universal biological macromolecule in nature,which has a variety of pharmacological activities and is mostly used in functional foods and therapeutic agents.The biological activity of polysaccharides depends on its chemical structure.In this paper,two dimensional nuclear magnetic resonance(2D NMR),X-ray diffraction,capillary electrophoresis combined with mass spectrometry(CE-MS),Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry(MALDI-TOF-MS),circular dichroism spectroscopy(CD),scanning electron microscopy(SEM),atomic force spectroscopy(AFM),differential scanning calorimetry(DSC)have been studied and molecular dynamics simulation were used to review the advanced structure of polysaccharides,and the future research work was prospected.