摘要
Background:Ceiba pentandra is a medicinal plant used as alternative therapy to control parasitic nematodes in livestock.Objective:This study aims to investigate anti-parasitic effect of aqueous stem back extract of Ceiba pentandra(L.)Gaertn through the evaluation of its anthelmintic,anti-inflammatory and analgesic activities.Methods:In vitro,the efficacy of aqueous extract(75 to 2400μg·mL−1)diluted in phosphate buffered saline(PBS)was tested against three stages of the life cycle of Haemonchus contortus through larval migration inhibition assay(LMIA),egg hatch assay(EHA),and adult worms motility inhibition assay(AMIA).In vivo,anti-inflammatory and antinociceptive properties of the aqueous extract(150 and 300 mg·kg−1)was evaluated on rodent using phlogistic and algic chemicals.Results:Significant inhibition activity(P<0.05)was obtained on EHA with the greatest inhibition of 46.63%obtained at 2400μg·mL−1.The plant treatment dramatically(P<0.05)inhibited L3 larval migration as compared to PBS.The highest inhibition rate was 53.33%at 1200μg·mL−1.Adding of polyvinylpolyrrolidone(PVPP)to the extract significantly(P<0.01)reduced at 38.6%the activity of the plant extract on larval migration compared to extract without PVPP.The Ceiba pentandra extract reduced(P<0.05)worm motility after 24 h post exposure as compared to control.In vitro,aqueous extract significantly(P<0.05)inhibited the paw inflammation induced by carragenine,with a significant(P<0.05)reduction of the number of abdominal contortions induced by acetic acid to 41.11%at 300 mg·kg−1 and the paw licking time induced by formaline in both phases to 57.22%and 63.59%at 300 mg·kg−1 likened to control.Conclusions:In vitro results suggest that,this plant possess anti-parasitic properties.Antinociceptive and anti-inflammatory effects of C.pentandra can contribute to its anti-parasitic property.
