黄藤水提物对AD果蝇疗效和寿命的影响

EFFECT OF WATER EXTRACT FROM FIBRAUREA RECISA ON THE EFFICACY AND LIFE SPAN IN AD DROSOPHILA MELANOGASTER

探讨黄藤水提物对AD果蝇疗效和寿命的影响。Elav-gal4基因型雌性果蝇和UAS-Aβ42基因型雄性果蝇进行杂交,收集三天内子代Elav-gal4>>UAS-Aβ42雄性果蝇,利用UAS-Aβ42/cyo雄性果蝇作为对照,实验组果蝇和对照组果蝇放置在浓度为0、0.5、1、2 mg/mL的黄藤水提物培养基中饲养0、7、14 d,观察爬管实验阳性的果蝇数量,并每天计数果蝇的死亡数直至全部果蝇死亡,计算果蝇平均寿命。结果表明黄藤水提物浓度为0.5 mg/mL饲养7 d和14 d时,与对照组相比,AD果蝇的神经行为学表现和存活时间有改善作用,但效果不显著(P>0.05);浓度增加到1 mg/mL时,实验组饲养7 d和14 d爬管实验阳性的果蝇数量与对照组相比均有所上升(P<0.05),并且延缓果蝇死亡时间,延长果蝇寿命(P<0.05);然而随着浓度的增加,饲养7 d和14 d的果蝇爬管行为学与对照组相比均有明显的抑制作用(P<0.05),果蝇的生存时间也显著减少(P<0.01)。此次实验还发现黄藤水提物在低浓度时治疗AD作用并不明显,但超过2 mg/mL时对AD果蝇爬管行为学和寿命均产生不利影响,因此只有在剂量和给药天数均合适的情况下才会产生积极作用。

To investigate the effect of Fibraurea recisa on neuroethology and life span of Drosophila melanogaster with overexpression of human Aβ42 protein.The Elav-gal4 genotype female and UAS-Aβ42 genotype were screened for hybridization,and the male offsprings of Elav-gal4>>UAS-Aβ42 within three days were collected.The male offsprings of UAS-Aβ42/cyo were set as the control,they were placed in 0,0.5,1,2 mg/mL of FR and fed for 0,7 and 14 days respectively.The number of the flies with positive climbing assay was observed,and their death number was counted every day until all flies died,and the average life span of the flies was calculated.The results showed that when the FR was 0.5 mg/mL for 7 and 14 days,compared with the control group,the climbing ability and life time of AD flies had no significant improvement.When the concentration was increased to 1 mg/mL,the number of the flies with positive climbing assay in the experimental group fed for 7 days and 14 days had increased compared with the control group,and it also delayed the death time of the flies,and there was a significant statistical difference compared with the control group(P<0.05).However,with the continuous increase of the concentrations,the climbing ability of flies fed for 7 days and 14 days was obviously inhibited compared with the control group,the life span of the flies was reduced.In this experiment,we found that the anti-AD effect of the FR was not obvious at low concentration,and it had a negative impact on the climbing ability and life span of AD flies when the concentrations exceeded a certain dosage,so only when the dosage and days of administration were appropriate it would have a positive effect.