摘要
利用超高效液相色谱-四级杆静电场轨道阱质谱联用技术对鬼针草的化学成分进行快速鉴定。采用Hypersil GOLD C18(100 mm×2.1 mm,1.9µm)色谱柱,以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,在加热电喷雾离子源(HESI)正负离子模式下对样品进行数据采集。采用高分辨质谱数据,结合对照品、文献、在线数据库,从鬼针草中鉴定出28种成分,包括10种黄酮类成分,6种氨基酸类成分,10种有机酸类成分,2种其它成分,其中壬二酸、7-羟基香豆素、没食子酸3种成分为首次在鬼针草中发现。该研究可为鬼针草的快速鉴定、质量控制方法建立和药效基础研究提供参考。
A method for rapid identification of the chemical components of Bidens by ultra-performance liquid chromatography-quadrupole orbitrap mass spectrometer(UPLC-Q-exactive orbitrap MS)was established.Thermo Hypersil GOLD C18(100 mm×2.1 mm,1.9µm)was used as the separation column,the mobile phase was acetonitrile and 0.1%formic acid solution.Mass spectrometric analysis was performed in the positive and negative heated electrospray ionization(HESI)modes.Based on the high-resolution mass spectrometry data,combined with previous literature,online databases and related standards mass spectrometry data,28 compounds were identified in Bidens,including 10 flavonoids,6 amino acids,10 organic acids and other components.Among them,azelaic acid,7-hydroxycoumarine and gallic acid were found in Bidens for the first time.This study provides a reference for rapid clinical identification,establishment of possible structure-activity relationships,and guidance for rational drug use through the comprehensive characterization of compounds in Bidens.
作者
王莉莉
刘月程
马祯
杨春林
WANG Lili;LIU Yuecheng;MA Zhen;YANG Chunlin(Shandong Provincial Hospital Affiliated to Shandong First Medical University,Jinan 250021,China;Shandong Academy of Chinese Medicine,Jinan 250014,China;Experimental Center,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)
出处
《化学分析计量》
CAS
2023年第5期11-17,共7页
Chemical Analysis And Meterage
基金
山东省医药卫生科技发展计划项目(202113030924)。
