BPA对活性污泥的毒性作用及生物修复

Experimental Studies on Toxicity of BPA to Activated Sludge and Related Bio-remediation

文章通过检测出水水质、污泥特性、微生物群落变化,分析双酚A(BPA)对SBR反应器中活性污泥微生物及功能的毒性作用,并通过红球菌R-001对活性污泥进行生物修复。结果表明,BPA减少了活性污泥的生物量,对EPS的分泌与积累产生负面影响,致使污泥沉降性能变差,COD、NH_(4)^(+)-N和TP的去除效率下降,并且这种损害性影响是持久性的。BPA还会抑制活性污泥脱氢酶活性,降低微生物的活菌比例,降低了Ferruginibacter、Polaromonas、Dechloromonas、Thauera和Nitrospira等参与有机物降解与代谢、EPS分泌和脱氮除磷的菌属丰度。红球菌R-001在短时间内可以增强BPA的去除效率(提升15%);恢复8.8%氨氮和22.7%的磷去除功能。

Aiming at probing into the toxic effects of BPA(bisphenol-A)on the microorganisms and functions of activated sludge in the SBR reactor,as well as the remediation using bio-augmentation technology,the bench-scale experiment was conducted,which involved detecting effluent water quality indicators,sludge characteristics and microbial community changes,and then the bio-remediation of activated sludge by use of Rhodococcus.As a result,findings of the experiment showed that BPA led to reduction of the biomass of activated sludge,negatively affecting the secretion and accumulation of EPS,resulting in poor sludge settling performance and reduced removal efficiency of COD,NH_(4)^(+)-N and TP,moreover the deteriorating influence was persistent;besides,BPA,as well,inhibited the dehydrogenase activity of activated sludge,diminishing the proportion of viable bacteria,thus reducing the abundance of bacteria and genera involved in the degradation and metabolism of organic matter,EPS secretion,and nitrogen and phosphorus removal,such as Ferruginibacter,Polaromonas,Dechloromonas,Thauera,and Nitrospira.However on the other hand,Rhodococcus R-001 can enhance the removal efficiency of BPA in a short time(increased by 15%),and can restore ammonia nitrogen by 8.8%and phosphorus removal function by 22.7%.