介孔氧化锆载药复合体的制备及性能测定

Synthesis of mesoporous zirconia implants as the biomedical molecules release system

目的:构建介孔氧化锆(m-ZrO2)负载及缓释盐酸米诺环素(MC-HCl)的复合体,观察其性质及体外药物缓释效果。方法:利用双有机溶剂分步挥发诱导自组装法创制具有介孔结构的氧化锆,并用于药物分子的可控负载。采用场发射扫描电镜分析所得样品的形貌以及介孔结构;利用MC-HCl对紫外-可见光的特征吸收,得出m-ZrO2对MC-HCl的吸附量及不同时间段的缓释量。结果:介孔氧化锆材料具有优异的吸附性能,37oC下对MC-HCl的吸附量达84.3 mg/g,是纯氧化锆的9倍。本工艺所得介孔氧化锆材料对MC-HCl的可控缓释效果明显,可将药物的释放时间由2 h增加到96 h。结论:建立了m-ZrO2负载及缓释MC-HCl复合体制备工艺,实现复合体在体外的可控负载及缓释,为预防种植体周围炎提供新思路。

Objective:To construct mesoporous zirconia implants as the biomedical molecules load-release system.Methods:A double-organic solvent evaporation induced self-assembly method was developed to synthesize mesoporous zirconia as a drug delivery system.Used field-emission scanning electron microscopy to observe the surface morphology and mesochannels of the as-prepared materials.The adsorption equilibrium linear equation was measured and compared by UV-visible spectrometer,and the adsorption amount of materials carrier to biomedical molecules(such as minocycline hydrochloride,MC-HCl)was calculated.The sustained release of materials was determined by indirect ultraviolet spectrophotometry.Results:Due to the enlarged surface areas of the mesochannels,mesoporous zirconia greatly improved the adsorption performance as the implants.The resultant implants showed a high adsorption capacity of 84.3 mg/g towards MC-HCl at 37℃,which was 9 times of bare zirconia.More importantly,the implants showed excellent drug sustained-release behavior,and the release time could be increased from 2 to 96 hours.Conclusion:Due to the excellent performance of load and sustained-release towards biomedical molecules,the mesoporous zirconia is expected to be a promising implant materials with a long-term antibacterial ability.