闭鞘姜叶绿体基因组特征及其系统发育分析

Analysis on Chloroplast Genome Characteristics and Phylogeny of Helenia speciosa

以闭鞘姜为试材,利用高通量测序技术对其进行叶绿体基因组测序、组装和注释,获得完整的叶绿体基因组。结果显示,闭鞘姜叶绿体基因组(GenBank No. OK 641589)大小为167 158 bp,具有典型的四分体结构,包括一个大单拷贝区(LSC,91 239 bp)、一个小单拷贝区(SSC,19 955 bp)和一对反向重复序列(IRa和IRb,各27 982 bp),总GC含量为36.3%;叶绿体基因组共编码了134个基因,包括88个蛋白质编码基因、38个tRNA基因和8个rRNA基因。闭鞘姜叶绿体基因组鉴定出50个散在重复序列以及70个简单重复序列(SSR),偏好使用AT结尾的密码子。系统发育分析表明,闭鞘姜与绿苞闭鞘姜(Costus viridis)的亲缘关系最为密切。

Using Helenia speciosa as test materials,the chloroplast genome was sequenced,assembled and annotated by high-throughput sequencing technology to obtain a complete chloroplast genome.The results showed that the chloroplast genome of Helenia speciosa(GenBank No.OK 641589)was 167158 bp in size and had a typical tetrad structure,including a large single-copy region(LSC,91239 bp),a small single-copy region(SSC,19955 bp)and a pair of reverse repeats(IRa and IRb,27982 bp each),with a total GC content of 36.3%,respectively.The chloroplast genome encoded a total of 134 genes,including 88 protein-coding genes,38 tRNA genes and 8 rRNA genes.Chloroplast genome preferred to use codons at the end of AT,50 dispersed repeating sequences and 70 SSR sites were identified from chloroplast genome of Helenia speciosa.Phylogenetic analysis showed that Hellenia speciosa was most closely related to Costus viridis.