原位调控基料碳源组分对水虻堆肥效率与碳氮固定效应的影响

Effect of the black soldier fly larvae composting efficiency and carbon-nitrogen fixation by in-situ regulation of carbon source composition

选择生物可利用度高的葡萄糖和生物利用度低的木屑作为碳源,花生粕作为氮源,配制总C/N为10、15、20、25、30,碳源难易利用程度为0%、25%、50%、75%、100%的25个水虻堆肥处理组,探究了不同总碳氮比和碳源难易利用程度下的水虻堆肥效率与碳氮素固定效应,基于相关性分析与正交偏最小二乘回归模型分析了调控碳氮固持的关键因子,并利用主成分分析法开展综合评价.结果表明:25个试验处理下,水虻虫体和虫粪产率分别为3.96%~23.06%和13.38%~89.99%;虫体碳含量为48.12%~60.31%,虫体氮含量为5.79%~9.85%,水虻从基料回收的碳、氮素分别为3.24%~29.58%和17.21%~52.69%;水虻堆肥体系碳与氮总固定率分别为40.69%~93.81%和38.06%~72.89%.易利用碳素有效率(ACC1)对水虻堆肥中虫体、虫粪的碳氮固定影响显著(VIP>1).总C/N为10,难易利用度碳源比为50%:50%时,水虻堆肥效率与体系总碳氮固定综合效果最佳.本研究证明了总C/N作为水虻堆肥营养基料配置指标上存在局限性,而原位调控基料碳源的生物利用度,可实现虫体高效生产与碳氮固定效能的协同平衡.

The glucose and rubber sawdust which is considered as high bioavailable and low bioavailable material,respectively,was set as carbon source.And the peanuts meal was used as the nitrogen source in the BSFL composting.25composting treatments with C/N of 10、15、20、25、30,and the ratio of available carbon component in 0%、25%、50%、75%、100%.Orthogonal partial least squares regression and relevance analysis were used to determine the key indices of composting and C-N fixation effect.BSFL composting effect between different treatments were comprehensively evaluated by principal component analysis.The results indicated that productivity of the larva and larva-cast were 3.96%~23.06%and 13.38%~89.99%,respectively.The content of C and N in larva of was 48.12%~60.31%and 5.79%~9.85%,respectively,of which 3.24%~29.58%and 17.21%~52.69%were recovered from the composting substrate,respectively.The total C and N fixation rates in BSLF composting were 40.69%~93.81%and 38.06%~72.89%,respectively.The proportion of carbon availability and active nitrogen components exhibits a significant effect on the C-N conversion rate of larva and larva cast(VIP>1).The ACC1(Available Activity Carbon 1)content and proportion should be more focus on in practical BSFL composting.The best comprehensive effect larva quality and C-N fixation were obtained in the total C/N of 10with the ratio of available to unavailable carbon component in 1:1.It is insufficient to conduct the BSFL composting only relying on substrate C/N without considering their availability.This study is significant for providing an efficient in-suit method in accurately regulating the bioavailability of the carbon source of the substrate,and finally result in enhancement of larva conversion efficiency and the C-N fixation.