对映-贝壳杉烷型二萜化合物Pseurata H对人食道癌ECA-109细胞生长的影响

Effects of an ent -kaurane diterpene pseurata H on the growth ofhuman esophageal cancer ECA-109 cells

为了研究对映-贝壳杉烷型二萜化合物Pseurata H对人食道癌ECA-109细胞的增殖抑制和诱导凋亡的作用,利用倒置显微镜观察法和CCK-8检测Pseurata H对ECA-109细胞生长的影响;利用考马斯亮蓝染色法和吖啶橙/溴化乙啶(AO/EB)双染法检测药物对细胞的张力纤维损伤和细胞核形态的影响.结果显示,用浓度分别为3.125μmol/L、6.25μmol/L、12.5μmol/L、25μmol/L的Pseurata H作用ECA-109细胞24 h后,ECA-109细胞生长均受到抑制,Pseurata H对ECA-109细胞的半抑制浓度值(IC 50)为3.22μmol/L,当浓度6.25μmol/L的药物作用细胞24 h后,细胞数量减少、形态开始变圆脱落、微丝减少且核仁凝结,随着浓度增加,现象越明显.由此可见,二萜化合物Pseurata H在体外能抑制食道癌ECA-109细胞生长,具有时间-剂量依赖效应.

To investigate the inhibitory effect of the ent-kaurane diterpenoid compound Pseurata H on the proliferation and induction apoptosis in human esophageal cancer ECA-109 cells.The viability of ECA-109 cell growth were detected by using inverted microscope and CCK-8.Coomassie brilliant blue staining and acridine orange/ethidium bromide(AO/EB)double staining method were used to detect the effects of Pseurata H on the tension fiber damage and nuclear morphology of ECA-109 cells.The results showed that the ECA-109 cells were inhibited by Pseurata H at concentrations as 3.125μmol/L,6.25μmol/L,12.5μmol/L and 25μmol/L for 24 hours,and the half maximal inhibitory concentration(IC 50)of Pseurata H on ECA-109 cells was 3.22μmol/L.The cell numbers decreased,their morphology began to become round and defluxionb,microfilaments decreased,and nucleoli condensed,after ECA-109 were treated with the concentration of 6.25μmol/L for 24 hours.As the concentration increases,the phenomenon became more obvious.It can be seen that the diterpenoid compound Pseurata H can inhibit the growth of esophageal cancer ECA-109 cells in vitro,with a time-dependent effect.