喜树转录因子CaERF1的克隆与生物信息学分析

Cloning and Bioinformatics Analysis of Transcription Factor CaERF1 from Camptotheca acuminata Decne

AP2/ERF (APETALA2/ethylene response factor)家族转录因子在调控植物生长以及次生代谢积累等方面具有重要功能。本研究从喜树叶片中克隆AP2/ERF家族转录因子,并利用生物信息学分析该转录因子序列与结构。结果表明,从喜树叶片中成功克隆了597 bp大小的转录因子,并命名为CaERF1,成功构建重组质粒pGMT-CaERF1;CaERF1蛋白由199个氨基酸组成,与拟南芥中ORA47具有较高同源性,且属于不稳定的亲水性蛋白质;二级结构以无规则卷曲占比最高,为68.84%,并在15~80氨基酸区域存在一个AP2结构功能域。该转录因子的克隆以及结构分析为进一步研究CaERF1的功能奠定基础,同时为研究AP2/ERF类转录因子调控喜树碱等次生代谢产物合成提供数据支持。

AP2/ERF(APETALA2/ethylene response factor)family transcription factors play an important role in regulating plant growth and secondary metabolic accumulation.In this study,an AP2/ERF family transcription factor was cloned from Camptotheca acuminata leaves,and the sequence and structure of the transcription factor were analyzed by bioinformatics.The results were as follows:The transcription factor CaERF1(597 bp)was successfully cloned from C.acuminata leaves and the recombinant plasmid pGMT-CaERF1 was constructed;The CaERF1 protein encoded a total of 199 amino acids,and it had high homology with ORA47 from Arabidopsis thaliana,and it was an unstable hydrophilic protein;The proportion of random coils in the secondary structure was the highest,accounting for 68.84%,and there was an AP2 structural functional domain in the 15~80 amino acid region.The cloning and structural analysis of this transcription factor provides a basis for further study of the function of CaERF1,and provide data support for the study of AP2/ERF transcription factors regulating the biosynthesis of secondary metabolites such as camptothecin.