基于立枯丝核菌侵染结缕草转录组数据的植物-病原菌互作分析

Analysis of Plant-pathogen Interaction Based on Transcriptome of Rhizoctonia solani Infecting Zoysia japonica

植物进化出复杂而精准的信号网络以调控寄主细胞响应病原菌侵染。为探究植物与病原菌互作过程的分子机制,本研究基于立枯丝核菌侵染结缕草的Zoysia·Unigene库与差异表达基因库,利用生物信息学分析主要生物代谢过程并筛选出结缕草与立枯丝核菌互作的分子过程,发现Ca2+调节与磷酸化两条路径与结缕草响应立枯丝核菌密切相关,且侵染24 h是互作的重要时间节点。本研究揭示结缕草与立枯丝核菌互作的分子机制,以期为草坪草在抗褐斑病的分子机制方面的研究提供理论依据。

Plants have evolved complex and precise signaling networks to regulate the response of host cells to pathogen infection.In order to explore the molecular mechanism of plant-pathogen interaction,bioinformatics was used to analyze the main biological metabolic processes and screen out the molecular processes of the interaction between Rhizoctonia solani and Zoysia japonica,basing on the transcriptome of Rhizoctonia solani infecting Zoysia japonica.The results showed that Ca?+regulation and phosphorylation pathways were closely related to the re-sponse of z.japonica to R.solani,and 24 h of infection was an important time node for the interaction.This study provides a theoretical basis for exploring the molecular mechanism of the Z.japonica-R.solani interaction.