转基因抗虫水稻HD1不同生长发育阶段cry1C*基因表达量

Expression of cry1C*Gene at Different Growth and Development Stages of Transgenic Pest-resistant Rice HD1

以水稻‘空育131’为受体,利用根瘤农杆菌介导法,创制了转cry1C*基因的抗虫水稻HD1-1等独立系。为了研究水稻HD1-1等在田间生长发育过程中cry1C*基因表达量及糙米Bt蛋白含量,将水稻HD1-1等独立系种植在田间,利用RT-qPCR方法检测分蘖期、抽穗期和灌浆期叶片、茎鞘及幼穗的cry1C*基因mRNA表达量,同时利用酶联免疫吸附法(ELISA)检测各器官及糙米Bt蛋白含量。分析cry1C*基因表达量不同生长发育阶段间的关系,分析糙米Bt蛋白含量与生长发育时cry1C*基因表达量的关系。结果显示,不同水稻独立系cry1C*基因表达量及糙米Bt蛋白含量明显不同,叶片、茎鞘及幼穗中cry1C*基因表达量不同生长发育阶段间往往呈正相关,cry1C*基因mRNA表达量和对应的Bt蛋白含量呈极显著的正相关,糙米Bt蛋白含量和各生长发育阶段各器官cry1C*基因表达量存在正相关趋势。水稻HD1 cry1C*基因表达量在不同生长发育阶段间及其和糙米Bt蛋白含量间呈正相关或正相关趋势。cry1C*基因转录水平越高,Bt蛋白含量越高。

HD1-1 and other independent lines of transgenic pest-resistant rice with cry1C*gene were created by A grobacterium tumefaciens mediated method with rice'Kongyul31'as receptor.Rice HD1-1 and other independent lines were planted in paddy field in order to research on expression of crylC*gene at different growth and develop-ment stages and Bt protein content(BPC)of brown rice in them.mRNA expression of cryIC*gene of leaf blade,sheath-culm and young panicle were detected with RT-qPCR at tillering stage,heading stage and filling stage.Meanwhile,BPC of these organs and BPC of brown rice were measured with enzyme-linked immunosorbent assay(ELISA).Correlations among different growth and development stages were analyzed in expression of crylC*gene,and relations was done between BPC of brown rice and expression of crylC*gene during growth and development period.The results showed that there was significant difference among rice independent lines in expression of crylC*gene and BPC of brown rice.There were often positive correlation among different growth and development stages in expression of crylC*gene of leaf blade,sheath-culm and young panicle.mRNA expression of crylC*gene was positively correlated to the corresponding BPC very significantly.It was shown that there was possibly positive correlation between BPC of brown rice and expression of crylC*gene of each organ at each stage.In expression of crylC*gene of rice HD1,there were significantly or possibly positive relations among different growth and development stages and between the ones and BPC of brown rice.The higher crylC*gene transcribed,the higher the BPC was.