细胞色素C氧化酶COX7B2在转移性卵巢癌的表达及临床意义

Expression of Cytochrome C Oxidase COX7B2 and Its Clinical Significance in Metastatic Ovarian Cancer

目的检测COX7B2在转移性卵巢癌中的表达,探究其表达模式,判断COX7B2对卵巢癌预后的影响,分析其作为卵巢癌诊断标志物的潜在价值.方法挖掘TCGA数据库中COX7B2的RNA表达数据,对邯郸市中心医院卵巢癌样本组织蜡块进行免疫组织化学染色,分析蛋白表达.结合生存数据进行临床分期和预后分析.分析COX7B2表达与免疫细胞浸润的相关性.结果COX7B2在卵巢癌组织中高表达,且转移性卵巢癌的表达更为显著.COX7B2的表达与患者组织学分级、病理分期、淋巴结转移、远端转移相关,与患者生存时间呈负相关.COX7B2对于卵巢癌的诊断具有较高的准确性.COX7B2抑制T细胞、NK细胞、DC细胞在肿瘤微环境中的浸润.结论COX7B2在卵巢癌中的高表达,影响患者的预后,COX7B2有望作为卵巢癌的诊断标志物,以及卵巢癌转移的特征性标志物.

Objective To detect the expression level of COX7B2 in metastatic ovarian cancer,explore its expression pattern,determine the effect of COx7B2 on the prognosis of ovarian cancer,and analyze its potential value as a diagnostic marker for ovarian cancer.Methods The RNA expression level of COX7B2 in ovarian cancer were analyzed in TCGA database.The expression of COx7B2 protein in ovarian cancer tissue samples from Handan Central Hospital was analyzed by immunohistochemical staining.Combined with the survival data,the clinical staging and prognosis were analyzed.The correlation between the expression of COX7B2 and the immune cell infiltration was analyzed.Results COX7B2 was highly expressed in ovarian cancer tissues especially in the tissues of metastatic ovarian cancer.The expression level of COX7B2 was correlated with the patient histological grade,pathological stage,lymph node metastasis,distal metastasis,and the patient survival time.The diagnosis of ovarian cancer by the expression of COx7B2 had a high accuracy.COX7B2 inhibited the infiltration of T cells,NK cells,and DC cells in the tumor microenvironment.Conclusion The COX7B2 is highly expressed in the ovarian cancer tissues,which affects the prognosis of patients.COX7B2 is expected to be served as a diagnostic marker for ovarian cancer,as well as a characteristic marker for the metastatic ovarian cancer.