RABL6高表达促进口腔鳞癌细胞的增殖、迁移和侵袭

Overexpression of RABL6 Promotes the Proliferation,Migration and Invasion of Oral Squamous Cell Carcinoma Cells

目的口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)是一种复发率高、转移率高、预后差的常见癌症,研究旨在深入了解RABL6在OSCC肿瘤发生和发展及预后中的作用.方法采用定量逆转录聚合酶链反应(qRT-PCR)和Western印迹检测37例冷冻临床样本和OSCC细胞中RABL6的表达.分析RABL6的表达及其与临床病理特征的相关性.通过CCK-8、克隆形成、流式细胞术、细胞划痕和Tran-swell实验,研究RABL6基因敲低对OSCC细胞增殖、凋亡和迁移能力的影响.结果与癌旁组织相比,RABL6在OSCC组织中高表达,与预后不良相关;与正常口腔角质形成细胞相比,RABL6在OSCC细胞中高表达;与si-NC组比较,敲低RABL6抑制了OSCC细胞的增殖、迁移和侵袭,还能诱导细胞凋亡.结论RABL6在OSCC中作为肿瘤癌基因发挥作用.提示可作为预测预后的潜在生物标志物,也是OSCC治疗的新靶点.

Objectivee Oral squamous cell carcinoma(OSCC)is a common cancer with high recurrence rate,high metastasis rate and poor prognosis.This study aims to further understand the role of RABL6 in the occurrence,development and prognosis of OSCC tumors.Methods The quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western blotting were used to detect the expression of RABL6 in 37 frozen clinical samples and OSCC cells.The correlation between RABL6 expression and clinicopathologic features was assayed.The effects of RABL6 gene knockdown on the proliferation,apoptosis and migration of OSCC cells were studied by CCK-8,colony formation,flow cytometry,wound healing and transwell assay.Results Compared with the normal adjacent tissues,the OSCC tissues have higher expression level of RABL6,which is associated with poor prognosis.Compared with the normal oral keratinocytes group,the OSCC cells have higher expression level of RABL6.Knockdown of RABL6 inhibited the proliferation,migration and invasion of OSCC cells and induced apoptosis when compared with the si-NC group.Conclusion RABL6 acts as a tumor oncogene in OSCC,which can be a potential biomarker for predicting prognosis and a new target for OSCC therapy.