滇重楼茎叶化学成分研究

Chemical constituents from stems and leaves of Paris polyphylla var.yunnanensis

目的研究滇重楼Paris polyphylla var.yunnanensis茎叶的化学成分。方法运用HPD100大孔吸附树脂、硅胶、Sephadex LH-20、ODS等多种柱色谱及制备液相色谱对滇重楼茎叶提取物进行分离纯化,通过核磁共振、质谱等波谱学方法并结合文献对分离得到的化合物进行结构鉴定。结果从滇重楼茎叶提取物中分离得到6个化合物,分别鉴定为7α-羟基偏诺皂苷元(1)、薯蓣皂苷元(2)、(25R)-螺甾-5-烯-3β,7β,21-三醇(3)、diosbulbisin C(4)、偏诺皂苷元-3-O-β-D-吡喃葡萄糖苷(5)、薯蓣皂苷元-3-O-β-D-吡喃葡萄糖苷(6)。结论化合物1为新化合物;化合物3、4为首次从该属植物中分离得到。

Objective To study the chemical constituents of the stems and leaves of Paris polyphylla var.yunnanensis.Methods Compounds were isolated and purified from the extract of the stems and leaves of P.polyphylla var.yunnanensis by various chromatographic techniques such as HPD100,silica gel,Sephadex LH-20,and ODS as well as by preparative HPLC.Their structures were identified by NMR,MS and other spectroscopic methods combined with literature.Results Six compounds were isolated from the extract of the stems and leaves of P.polyphylla var.yunnanensis,and identified as 7α-hydroxyl pennogenin(1),diosgenin(2),(25R)-spirost-5-ene-3β,7β,21-triol(3),diosbulbisin C(4),pennogenin 3-O-β-D-glucopyranoside(5),and diosgenin 3-O-β-D-glucopyranoside(6).Conclusion Compound 1 is a new compound;Compounds 3 and 4 are isolated from this genus for the first time.