猪心血丹参性状特征与内在品质相关性研究

Research on correlation between morphological characteristics and internal quality of Salvia miltiorrhiza processed by porcine cardiac blood

目的探究猪心血丹参(Salvia miltiorrhiza processed by porcine cardiac blood)性状特征与内在品质之间的关系,快速评价猪心血丹参的质量。方法基于分光测色等技术对猪心血丹参性状特征建立客观量化方法,结合HPLC技术建立二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA4个二萜类成分及丹参素钠、迷迭香酸、紫草酸、丹酚酸B 4个酚酸类成分的多成分含量测定方法,并对上述表(性状特征)、里(内在品质)指标建立参考范围,进行了相关性分析,建立线性回归方程,并进行真实世界样品验证。结果酚酸类成分与横截面直径显著正相关,丹参酮类成分与粉末a^(*)值显著正相关,水分值与粉末L^(*)值显著负相关,建立了7个内外关联的线性回归方程,验证证明相对误差小于10%,可对猪心血丹参质量进行快速评价。结论分析总结了猪心血丹参性状特征与内在品质的相关性,为猪心血丹参饮片快速评价奠定基础,并为传统“辨状论质”理论提供支撑。

Objective To investigate the relationship between the appearance and composition of Danshen(Salvia miltiorrhiza)processed by porcine cardiac blood,to quickly evaluate the quality of S.miltiorrhiza processed by porcine cardiac blood.Methods Spectrophophotometric colorimetry was used to objectively quantify the morphological characteristics of the samples,and HPLC was used to establish multi-component content determination methods for sodium danshensu,rosmarinic acid,lithospermic acid,lithospermic acid B,dihydrotanshinone I,cryptotanshinone,tanshinone I,tanshinone IIA,and establish reference ranges for the indices listed above.In addition,a linear regression equation was developed and real-world sample validation was conducted to determine the correlation between appearance and composition.Results The phenolic acid components were significantly and positively related with the cross-sectional diameter.The tanshinone components were significantly and positively correlated with the powder's a^(*),and the water content was significantly and negatively associated with the power’s L^(*).Seven appropriate forecasting linear regression equations were derived.The validation showed that the relative error was less than 10%,which can be used for rapid evaluation of the quality of S.miltiorrhiza processed by porcine cardiac blood.The relationship between the appearance and composition of S.miltiorrhiza processed by porcine cardiac blood was investigated and summarized,laying the foundations for a rapid evaluation of quality as well as offering theoretical backing for the traditional theory of“judging quality by traits”.