mTOR抑制剂对癫痫大鼠免疫微环境的影响

Influence of mTOR Inhibitor Intervention on Immune Microenvironment in Epileptic Rats

目的探讨mTOR抑制剂对癫痫大鼠血液和脑组织免疫微环境的影响。方法选用40只癫痫大鼠,随机分为4组,每组10只,空白对照组(生理盐水干预),不同剂量mT OR抑制剂(雷帕霉素)干预组:低剂量组[1 mg/(kg·d)],中剂量组[2 mg/(kg·d)],高剂量组[4 mg/(kg·d)]。分别进行为期8周的干预,8周后处死大鼠,保留大鼠血清及脑组织样本。经ELISA法检测大鼠血清IL-2、IL-10和IFN-γ水平。经流式细胞技术检测大鼠脑组织的T细胞亚群。结果癫痫大鼠外周血IL-2、IL-10表达差异无统计学意义(P>0.05),IFN-γ表达差异有统计学意义(P<0.05)。脑组织中T淋巴细胞表达差异有统计学意义(P<0.05),随着给药剂量增加,T淋巴细胞含量有降低趋势,但始终高于空白组。结论雷帕霉素对癫痫大鼠的免疫微环境有调节作用,对脑组织起保护作用。

Objective To determine the influence of rapamycin intervention on epileptic rats blood and the brain tissue immune microenvironment.Methods A total of 40 epileptic rats were used in the study and randomly divided into 4 groups(n=10).The blank control group with normal saline intervention,and with different doses of rapamycin:low-dose group[1 mg/(kg·d)],medium-dose group[2 mg/(kg·d)],high-dose group[4 mg/(kg·d)].Rats in the four groups went through intervention for 8 weeks.After 8 weeks,the rats were sacrificed,rat serum and brain tissue samples were reserved.The ELISA method was used to test peripheral blood(PB),IL-2,IFN-γand IL-10 levels.Flow cytometry was used to test the state of T-cell subsets.Results There was no significant difference in levels of serum IL-2 and IL-10(P>0.05),but was significant difference in levels of serum IFN-γ(P<0.05),and the brain tissue T-cell subsets(P<0.05).With the increase of dose,T lymphocyte level had a tendency to decrease,but was always higher than that of blank group.Conclusion Rapamycin has regulatory effect on epileptic rats of immune microenvironment,and has protection on brain tissue.