黄绿卷毛菇对其生境土壤微生物群落结构的影响

Effects of Floccularia luteovirens on the microbial communitystructure in its habitat soil

黄绿卷毛菇Floccularia luteovirens是青藏高原特有的珍稀食药用真菌,具有较高的经济价值和生态功能,其对土壤微生物群落的调控及稳定具有促进作用。本研究采用IlluminaMiSeq测序技术,对9份黄绿卷毛菇菌窝土壤(F)及9份周围无菇土壤(CK)微生物群落组成和多样性进行分析。结果表明,黄绿卷毛菇生境土壤中共获得25门、61纲、99目、174科、442属细菌。与周围无菇土壤相比,黄绿卷毛菇增加了土壤细菌的多样性(Shannon指数6.55)和丰富度(Ace指数8104.25);变形菌门Proteobacteria(F:49.83%,CK:37.85%),鞘脂单胞菌属Sphingomonas(F:16.17%,CK:13.28%)的相对多度均显著升高。PICRUST功能分析表明,黄绿卷毛菇生境土壤中代谢功能系统为细菌群落的主要通路,在F组和CK组中所占比例分别为38.66%和13.08%。生境土壤中共获得真菌15门、38纲、93目、204科、304属。与周围无菇土壤相比,黄绿卷毛菇降低了土壤真菌的多样性(Shannon指数1.06)和丰富度(Ace指数1269.88);担子菌门Basidiomycota的相对多度显著升高(F:87.27%,CK:36.06%),而子囊菌门Ascomycota的相对多度显著降低(F:7.05%,CK:43.28%);卷毛菇属Floccularia的相对多度为85.76%,为绝对优势属。FunGuild功能预测表明土壤真菌以共生营养型为主,其次为腐生营养型。黄绿卷毛菇对其生境土壤微生物的驱动机制亟待研究。

Floccularia luteovirens is a medicinal and edible fungus found only in the Qinghai Tibet Plateau.It has a high economic value and ecological function of regulating and stabilizing the soil microbial community.The composition and diversity of microbial communities in 9 samples of F.luteovirens nest soil(F)and 9 samples of surrounding soil(CK)were examined using Illumina MiSeq sequencing techniques.Bacteria belonging to 25 phyla,61 classes,99 orders,174 families,and 442 genera were found in the habitat soil of F.luteovirens.The presence of F.luteovirens resulted in the increase of the diversity(Shannon index 6.55)and richness(Ace index 8104.25)of soil bacteria as compared with diversity and richness of bacteria in the surrounding soil.The relative abundance of Proteobacteria(F:49.83%,CK:37.85%)and Sphingomonas(F:16.17%,CK:13.28%)was significantly increased.The metabolic function system in the soil of F.luteovirens habitat was the main pathway of the bacterial community,accounting for 38.66%and 13.08%in the F and CK groups,respectively,according to PICRUST functional analysis.In total,15 phyla,38 classes,93 orders,204 families,and 304 genera of fungi were obtained from the habitat soil.Compared with the surrounding soil,the presence of F.luteovirens decreased soil fungal diversity(Shannon index 1.06)and richness(Ace index 1269.88).The relative abundance of Basidiomycota significantly increased(F:87.27%,CK:36.06%),while that of Ascomycota decreased significantly(F:7.05%,CK:43.28%).The relative abundance of Floccularia was 85.76%,being absolutely dominant.The functional prediction of FunGuild showed that soil fungi were mainly symbiotrophic,secondarily saprotrophic.The driving mechanism of F.luteovirens for microorganisms in the habitat soil needs further study.