超高效液相色谱-电喷雾串联四级杆质谱鉴定灵芝中脂质成分

Identification of lipid components in Ganoderma lingzhi byultra high performance liquid chromatography-electrospraytandem quadrupole mass spectrometry

脂质是灵芝重要活性成分之一,但目前对灵芝胞内脂质成分的构成研究甚少。本研究采用UPLC-ESI-MS/MS技术,对灵芝发酵菌体的胞内脂质构成进行分析。结果显示,灵芝细胞中共鉴定到296种脂质,其中甘油酯112种、磷脂148种、鞘脂34种和甾醇2种;甘油酯和磷脂分别占总脂质的44.70%和38.06%,鞘脂和甾醇分别占总脂质的17.08%和0.16%。分析甘油酯中主要成分为甘油三酯,占甘油酯总含量的67.36%;磷脂中主要成分为磷脂酰乙醇胺,占磷脂总含量的62.64%;鞘脂中主要成分为神经酰胺,占鞘脂总量的60.33%;此外,本研究检测出27种游离脂肪酸,其中20种为不饱和脂肪酸,相对含量为65.59%;7种为饱和脂肪酸,相对含量为34.41%。本研究系统性地分析了灵芝细胞中的脂质构成,为进一步开展灵芝细胞中脂质相关研究奠定基础。

Lipids are one of the most important active components of Ganoderma lingzhi.However,there are few studies on the composition of intracellular lipids in G.lingzhi.Here,the intracellular lipid composition was analyzed by UPLC-ESI-MS/MS technique from G.lingzhi mycelium cell.The results showed that 296 kinds of lipids were identified in G.lingzhi cells,including 112 glycerides,148 phospholipids,34 sphingolipids and 2 sterols.Glycerides and phospholipids account for 44.70%and 38.06%of the total lipids respectively.Sphingolipids and sterols account for 17.08%and 0.16%respectively.The main component of glycerides is triglyceride,accounting for 67.36%of the total glycerides.The main component of phospholipids is phosphatidylethanolamine,accounting for 62.64%of the total phospholipids.The main component of sphingolipids is ceramide,accounting for 60.33%of the total sphingolipids.In addition,27 fatty acids were detected,in which 20 were unsaturated fatty acids,with a relative content of 65.59%,and 7 were saturated fatty acids,with a relative content of 34.41%.This study laid a foundation for further study of lipids in G.lingzhi cells.