大推天河水对内毒素性发热幼兔的解热作用机制研究

Study on the antipyretic mechanism of large pushing Tianheshui for young rabbits with endotoxin-induced fever

目的:观察大推天河水推拿手法干预内毒素致热幼兔对其体温的影响,并探讨其解热机制。方法:普通级雄性新西兰幼兔40只,适应性饲养7 d,筛选出符合标准的32只幼兔,按随机数字表法随机分为正常组、模型组、大推天河水组和布洛芬组,每组8只。模型组、大推天河水组和布洛芬组通过耳缘静脉注射脂多糖建立内毒素致热模型。大推天河水组动物分别于造模后1.5 h及2.5 h进行推拿干预;布洛芬组在造模后1.5 h予以布洛芬混悬液灌胃。观测致热幼兔造模后体温动态变化;应用酶联免疫吸附法测定下丘脑体温中枢正调节介质前列腺素(PG)E2、环磷酸腺苷(cAMP)和负调节介质精氨酸加压素(AVP)及α黑色素细胞刺激素(α-MSH)的含量变化。结果:模型组幼兔造模后体温在0.5~4.0 h、5.0 h及5.5 h均显著高于正常组(P<0.01);模型组存在2个明显的发热高峰,分别在造模后1.5 h和3.0 h,其中最高峰在1.5 h。与模型组比较,大推天河水组与布洛芬组干预0.5 h后体温显著下降(P<0.05)。与正常组比较,模型组下丘脑PGE2及cAMP含量明显升高(P<0.05),下丘脑AVP及α-MSH含量显著降低(P<0.01)。与模型组比较,大推天河水组及布洛芬组下丘脑中PGE2及cAMP含量显著降低(P<0.01),AVP及α-MSH含量显著升高(P<0.01)。布洛芬组和大推天河水组的下丘脑PGE2、cAMP、AVP及α-MSH含量差异均无统计学意义(P>0.05)。结论:大推天河水推拿手法对内毒素致热幼兔有明显的退热作用,其作用机理可能与抑制下丘脑体温正调节介质PGE2及cAMP水平和促进负调节介质AVP及α-MSH的释放有关。

Objective:To observe the effect of large pushing Tianheshui manipulation intervention on the body temperature of young rabbits with endotoxin-induced fever and discuss its antipyretic mechanism.Methods:Thirty-two young rabbits meeting the standards were selected from 40 ordinary young male New Zealand rabbits after being adapted for 7 d,and randomly divided into a normal group,a model group,a large pushing Tianheshui group,and an ibuprofen group according to the random number table method,with 8 rabbits in each group.Rabbits in the model group,the large pushing Tianheshui group,and the ibuprofen group were subjected to preparing the endotoxin-induced fever model by intravenous lipopolysaccharide from the marginal ear vein.Rabbits in the large pushing Tianheshui group received Tuina(Chinese therapeutic massage)manipulation intervention 1.5 h and 2.5 h after modeling,respectively.Rabbits in the ibuprofen group were intragastrically given ibuprofen suspension 1.5 h after modeling.The dynamic changes in body temperature were observed for the young rabbits after fever modeling.Enzyme-linked immunosorbent assay was used to determine the content changes in positive mediators of hypothalamic body temperature,including prostaglandin(PG)E2 and cyclic adenosine monophosphate(cAMP),as well as negative mediators of hypothalamic body temperature,including arginine vasopressin(AVP)andα-melanocyte stimulating hormone(α-MSH).Results:The body temperature of the young rabbits in the model group was significantly higher than that in the normal group at 0.5-4.0 h,5.0 h,and 5.5 h after modeling(P<0.01),showing two obvious fever peaks in the model group at 1.5 h and 3.0 h after modeling,respectively,with the highest peak at 1.5 h.Compared with the model group,body temperatures of the large pushing Tianheshui group and the ibuprofen group decreased significantly after 0.5 h of intervention(P<0.05).Compared with the normal group,the contents of PGE2 and cAMP were significantly increased(P<0.05),and the contents of AVP andα-MSH were significantly decreased(P<0.01)in the hypothalamus of the model group.Compared with the model group,the contents of PGE2 and cAMP were significantly decreased(P<0.01),and the levels of AVP andα-MSH were significantly increased(P<0.01)in the hypothalamus in the large pushing Tianheshui group and the ibuprofen group.There were no significant differences in the PGE2,cAMP,AVP,andα-MSH contents in the hypothalamus between the ibuprofen group and the large pushing Tianheshui group(P>0.05).Conclusion:Large pushing Tianheshui manipulation has a significant antipyretic effect on endotoxin-induced fever in young rabbits.The mechanism may be related to inhibiting the positive regulators(PGE2 and cAMP)and promoting the negative regulators(AVP andα-MSH)of hypothalamic control of body temperature.