基于UPLC-QTOF/MS的茵陈蒿汤体外成分鉴定和急性肝损伤防护作用入血成分研究

Identification of constituents in vitro and blood-absorbed ingredients of protective effect on acute liver injury from Yin Chen Hao decoction based on UPLC-QTOF/MS

鉴定茵陈蒿汤体外活性成分和体内入血成分,为探究其对急性肝损伤防护作用和防治机制提供了科学依据。利用超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF/MS)技术,鉴定茵陈蒿汤水煎液及口服预防给药14天后,刀豆蛋白A(con-A)所致急性肝损伤小鼠的血清样本(本研究中所有实验动物的使用获得海军军医大学伦理委员会批准,批号19YF1459400)中的化学成分。初步鉴定茵陈蒿汤水煎液含90个化合物,主要为黄酮类、萜类、鞣质类、醌类等,血清样本中含5个原形成分,包括大黄酚、脱氧土大黄苷-8-O-没食子酸盐、玉叶金花苷酸、7-甲氧基-香豆素、大黄素。本文所建立的UPLC-QTOF/MS方法能高效灵敏地鉴别茵陈蒿汤的体外成分及其入血成分,初步阐明茵陈蒿汤保肝作用的药效物质基础,可为茵陈蒿汤的质量标志物筛选和药理作用研究提供科学依据。

To identify the active constituents in vitro and blood-absorbed ingredients in vivo from Yin Chen Hao decoction provides scientific evidence for probing its prevention and treatment mechanism on acute liver injury.An ultrahigh performance liquid chromatography quadrupole-time of flight-mass spectrometry(UPLCQTOF/MS)method was applied for analysis of Yin Chen Hao decoction and the serum samples of mice with con-A induced acute liver injury after preventive oral administration for 14 days(the use of all laboratory animals in this study was approved by the Ethics Committee of the Naval Medical University,19YF1459400).A total of 90 chemical constituents were identified from Yin Chen Hao decoction,mainly were flavonoids,terpenoids,tannins,quinones.5 prototype compounds were identified in the serum,including chrysophanol,deoxyrhapontin-8-Ogallate,mussaenosidic acid,herniarin,emodin.The established UPLC-QTOF/MS method could efficiently and sensitively identify the constituents in vitro and blood-absorbed ingredients of Yin Chen Hao decoction,primarily clarify the material basis of its hepatoprotective effect,and provided a scientific basis for the quality marker selection and the pharmacodynamic material basis research on the decoction.