决明GSK基因家族全基因组鉴定及其表达分析

Genome-wide identification of GSK gene family in Senna tora L.and expression analyses

糖原合成激酶3(glycogen synthase kinase 3/SHAGGY-like kinase,GSK3)在调节植物生长发育和胁迫响应方面发挥重要作用。为揭示药用植物决明(Senna tora L.)GSK家族成员特性,本研究基于其全基因组数据,结合生物信息学及基因表达研究方法,开展决明GSKs基因鉴定及表达分析。共鉴定到9个StoSKs基因家族成员,均具有GSK特征激酶结构域,9个成员分布在6条染色体上,编码氨基酸长度为465~943 aa,蛋白分子质量介于33.57~88.83 kDa,平均等电点为8.2。StoSKs基因家族分为4个进化分支,同一进化分支中的StoSKs之间具有相同的外显子/内含子结构及保守基序。StoSKs家族成员扩张主要源于片段重复事件,与大豆(Glycine max)、蒺藜苜蓿(Medicagotruncatula)、拟南芥(Arabidopsisthaliana)、水稻(Oryzasativa)分别存在17、11、8和7对共线基因对。StoSKs启动子区域多含有与胁迫刺激、生长发育、激素诱导相关的响应元件。转录组数据分析发现StoSKs在不同组织中均有表达,在根中表达水平最高。qRT-PCR(quantitative real-time PCR)分析表明,不同进化分支的StoSKs表现出协同响应光照的表达模式,多数StoSKs能快速响应NaCl胁迫处理,表达显著上调。研究结果为下一步进行决明GSK基因家族的生物学功能分析提供基础。

Glycogen synthase kinase 3/SHAGGY-like kinase(GSK3)proteins play important roles in regulating plant growth,development,and stress response.In order to reveal the characteristics of GSK family members in the medicinal plant Senna tora L.,in this study,we conducted the identification and expression analyses of GSKs in S.tora based on its whole genome data,combined with bioinformatics and gene expression research methods.The results showed that a total of nine S.tora GSK genes were identified,all of which contained the GSK characteristic kinase domains.All members were distributed on six chromosomes,the encoding amino acid length ranged from 465 to 943 aa,the protein molecular weight was from 33.57 to 88.83 kDa,and the average isoelectric point was 8.2.The StoSKs were divided into four evolutionary branches,and the StoSKs in the same evolutionary branch shared the same exon/intron structure and conserved motifs.The expansion of the StoSKs gene family was mainly due to segment duplication events,and there were 17,11,8 and 7 pairs of collinear genes with Glycine max,Medicago truncatula,Arabidopsis thaliana and Oryza sativa,respectively.The promoter regions of StoSKs mostly contained responses elements related to stress stimulation,growth and development,and hormone induction.Transcriptome data analysis showed that StoSKs were expressed in different tissues,with the highest expression level in roots.Quantitative real-time PCR(qRT-PCR)analysis indicated that StoSKs in different evolutionary branches displayed a synergistic expression pattern response to light,and most of StoSKs could rapidly respond to NaCl stress with significantly up-regulated expression.All the results provide a basis for further analysis of the biological functions of the GSKs gene family in S.tora.