Cysteine-activated fluorescence/photoacoustic integrated probe for non-invasive diagnosis of drug-induced liver injury

Hepatotoxicity is a serious problem faced by clinical drugs,and long-term administration or overdose may lead to liver failure and even death of patients.Therefore,developing a reliable detection method for the early diagnosis and therapy of drug-induced liver injury(DILI)has significant meaning.Nearinfrared fluorescence(NIRF)and photoacoustic(PA)dual-modality tomography probes can be used for imaging with high sensitivity and high-resolution of disease-related markers in deep tissues.Here,we developed a novel Cys-activated NIRF and PA dual-modality imaging probe(CDR)for early diagnosis of DILI,for the first time.The organic molecular probe CDR could respond rapidly to Cys,resulting in the absorption peak red-shifted from 560 nm to 725 nm,which also leads to the activation of the PA725signal and NIRF765signal.In addition,the new probe CDR could be used for NIRF/PA imaging of exogenous and endogenous Cys level in live cells and mice.More importantly,CDR has also been successfully used for in situ detection of Cys in early DILI mice and evaluate the therapeutic effect of NAC.Therefore,the CDR might become a powerful tool to research the physiological effect of Cys and evaluate the degree of DILI.