不同产地和树龄厚朴样本姜炙前后化学成分的定性定量分析

Chemical components of Magnoliae Officinalis Cortex of different origins and with different tree ages before and after being processed with ginger juice:a qualitative and quantitative analysis

为研究姜炙对厚朴化学成分的影响,采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱技术,对厚朴姜炙前后的化学成分进行定性分析;采用超高效液相色谱技术,考察姜炙对厚朴8种主要成分含量的影响。采用正、负离子扫描模式,共鉴定/推测出厚朴/姜厚朴中174个化合物;厚朴姜炙后,酚苷类化合物峰面积多上升,苯乙醇苷类化合物峰面积多下降,新木脂素类、氧新木脂素类、其他木脂素类、生物碱类化合物变化趋势不一,萜类木脂素类化合物峰面积未发生明显变化;从姜厚朴中检出姜辣素和二苯基庚烷类化合物。含量测定结果显示,厚朴姜炙后,紫丁香苷、木兰苷A、木兰苷B的含量显著降低,木兰花碱、木兰箭毒碱、和厚朴酚、obovatol、厚朴酚含量无显著变化。该研究采用超高效液相色谱、超高效液相色谱-质谱联用技术,对不同树龄及不同产地厚朴在姜炙前后的化学成分变化进行了较全面的考察,对各类化合物的变化规律进行了总结,为后续姜厚朴药效物质基础研究提供了实验数据。

This study aimed to investigate the impact of ginger juice on chemical profile of Magnoliae Officinalis Cortex(MOC)when they were processed together.Ultra-high-performance liquid chromatography coupled to quadrupole-orbitrap high-resolution mass spectrometry(UHPLC-Q-Orbitrap HRMS)was used for qualitative analysis of the chemical component of MOC samples before and after being processed with ginger juice.UPLC was performed to observe the content variation of eight main components in processed MOC.A total of 174 compounds were identified or tentatively deduced from processed and unprocessed MOC samples according to MS data obtained in positive and negative ion mode.After MOC was processed with ginger juice,the peak areas of most phenolics increased,while the peak areas of most phenylethanoid glycosides decreased;as for neolignans,oxyneolignans,other lignans and alkaloids,changes in the peak area were variable,and the peak areas of terpenoid-lignans varied little.Additionally,gingerols and diarylheptanoids were only detected in the processed MOC sample.The contents of syringin,magnoloside A,and magnoloside B decreased significantly in the processed MOC sample while no significant difference was observed in the contents of magnoflorine,magnocurarine,honokiol,obovatol,and magnolol.This study comprehensively explored the content variation of chemical components in processed and unprocessed MOC samples derived from different regions and with different tree ages using UPLC and UHPLC-Q-Orbitrap HRMS,and summarized the variation characteristics of various compounds.The results provide a data foundation for further research on pharmacodynamic substances of MOC processed with ginger juice.