Collagen1和Collagen3在牦牛肺纤维化组织中的表达研究

Expression of Collagen1 and Collagen3 in Pulmonary Fibrosis Lung Tissues of Yak

为探讨Collagen1和Collagen3在牦牛肺纤维化组织中的表达,阐明Collagen1和Collagen3在牦牛肺纤维化进程中发挥的作用,采集牦牛正常肺组织和发生纤维化的肺组织,将其分为对照组和试验组,通过HE染色、Masson染色及透射电镜观察肺超微结构的病理变化及纤维化状态;利用实时荧光定量PCR(qRT-PCR)、蛋白免疫印迹(WB)、免疫组化和免疫荧光染色法检测和定位Collagen1、Collagen3基因和蛋白的表达情况。结果显示,试照组牦牛肺组织结构完整,肺泡隔无增厚,支气管管腔和肺泡腔内均无炎性渗出物;试验组肺组织可见片状坏死,胞核碎裂溶解,重度出血,大范围肺水肿。Collagen1在试验组中的表达量高于对照组,而Collagen3在对照组中的表达量高于试验组。在试验组中,Collagen1和Collagen3蛋白大量增生,在肺泡隔中有大量分布,其余分布位置与肺对照组基本一致,但表达都高于对照组。综上所述,Collagen1和Collagen3在牦牛发生肺纤维化的过程中发挥着重要作用,Collagen1和Collagen3的相对表达量在诊断牦牛纤维化肺炎中具有一定的参考价值。

To investigate the expression of Collagen1 and Collagen3 in pulmonary fibrosis tissue of yak and clarify the roles of Collagen1 and Collagen3 in pulmonary fibrosis process of yak.The normal and fibrosis lung tissue of yak were collected and divided into control group and experimental group.HE staining,Masson staining and transmission electron microscope were used to observe the pathological changes of lung ultrastructure and fibrosis state;qRT-PCR,Western-blot,immunohistochemistry and immunofluorescence staining were used to detect the expression of Collagen1 and Collagen3 genes and proteins in groups.The results showed that the yak lung tissue structure of the control group was intact,the alveolar septum was normal and there was no inflammatory exudate in bronchial lumen and alveolar cavity.while in experimental group,lung tissue showed necrosis,nuclear fragmentation and dissolution,severe hemorrhage and extensive pulmonary edema.The expression of Collagen1 in experimental group is higher,while the expression of Collagen3 is lower than that in control group.In experimental group,Collagen1 and Collagen3 proteins were proliferated massively and distributed in alveolar septum extensively.The other distributions were basically the same as control group but stronger than in control group.In conclusion,Collagen1 and Collagen3 play important roles in yak pulmonary fibrosis.