H7N9和H1N1流感病毒感染BV2细胞的差异表达miRNA初步分析

Preliminary Analysis of MiRNA Expression in BV2 Cells Infected with H7N9 and H1N1 Influenza Viruses

筛选鉴定H7N9病毒感染BV2细胞的特异聚类microRNA(miRNA)并初步探讨这些miRNA的可能致病机制。H7N9和H1N1流感病毒感染BV2细胞,分别收集12 h、24 h和48 h的细胞并提取总RNA。并利用高通量测序技术进行miRNA测序,同时比较鉴定不同病毒特异性miRNA。筛选出了10个H7N9病毒特异聚类miRNA,其中有3个miRNA被miRBase数据库所收录。这些特异聚类miRNA调控诸多信号通路的信号传导,比如Ras信号通路、PI3K-Akt信号通路、MAPK信号通路、轴突导向和癌症相关基因等。该研究为miRNA调控H7N9禽流感病毒的致病机制提供了科学基础。

To screen and identify the specific clustered miRNAs of H7N9 infected BV2 cells and to preliminarily explore the possible pathogenesis of these miRNAs.Cells were collected at 12 h,24 h and 48 h respectively and total RNA was extracted.High throughput sequencing technology was used to sequence miRNAs,and different virus-specific miRNAs were compared and identified.Ten H7N9 virus-specific clustered miRNAs were screened,three of which were included in the miRBase database.The specific clustered miRNA regulated signal transduction of many signal pathways,such as Ras signal pathway,PI3K Akt signal pathway,MAPK signal pathway,axon guidance and cancer related genes.This study provided a scientific basis for research on the function of miRNAs in regulating the pathogenic mechanisms of H7N9 avian influenza virus.