兰州百合内生放线菌Streptomyces sp.LRE-541次级代谢物抗胃癌活性研究

Antigastric cancer activity of Streptomyces sp.LRE-541 secondary metabolite from Lilium davidii var.unicolor Cotton

为研究兰州百合(Lilium davidii var.unicolor Cotton)根部内生放线菌Streptomyces sp.LRE-541的次级代谢产物及抗胃癌活性,利用高效液相色谱法(HPLC)、核磁共振(NMR)等方法对该菌株次级代谢产物进行分离纯化及鉴定;以胃癌细胞HGC-803为研究对象,探讨代谢产物对细胞增殖细胞凋亡和细胞迁移能力的影响。结果从其发酵液中获得两个蒽醌类化合物,鉴定为4-Deoxy-ε-pyrromycinone(1)和epsilon-pyrromycinone(2)。化合物1和化合物2对胃癌细胞HGC-803的IC 50分别为(25.24±1.23)μg/mL和(17.29±2.28)μg/mL;在相同处理下,与空白对照组相比,15、25μg/mL的化合物1组和10、20μg/mL的化合物2组癌细胞增殖率、细胞凋亡率和细胞划痕愈合率明显下降,差异具有统计学意义(P<0.05或P<0.01)。表明这两种化合物都可抑制胃癌HGC-803细胞增殖和迁移能力,促进细胞凋亡,有成为抗胃癌药物或前体药物的潜力。

In order to study the secondary metabolites of Streptomyces sp.LRE-541 from the roots of Lilium davidii var.unicolor Cotton and its antigastric cancer activity,high performance liquid chromatography(HPLC),nuclear magnetic resonance(NMR)and other methods were used to isolate,purify and identify the secondary metabolites.The effects of metabolites on cell proliferation,apoptosis and migration of gastric cancer cell HGC-803 were investigated.Two anthraquinones were identified as 4-deoxy-ε-pyrromycinone(1)and epsilon-pyrromycinone(2).The IC 50 of compound 1 and compound 2 against gastric cancer cell of HGC-803 were(25.24±1.23)μg/mL and(17.29±2.28)μg/mL,respectively.Under the same treatment,compared with blank control group,the proliferation rate,apoptosis rate and scratch healing rate of cancer cells in 15 and 25μg/mL of compound 1 and 10 and 20μg/mL of compound 2 groups were significantly decreased,the differences were statistically significant(P<0.05 or P<0.01).These results indicated that the two compounds could inhibit the proliferation and migration of gastric cancer of HGC-803 cells and promote the apoptosis of gastric cancer cells,which might be potential as anti-gastric cancer drugs or precursors.