摘要
N6-甲基腺嘌呤(N6-methyladenosine,m^(6)A)是mRNA中含量最丰富的化学修饰之一,在各种生理病理过程中发挥关键性的作用。m^(6)A修饰主要位于mRNA终止密码子附近和长的内部外显子上,然而导致这一特异性分布的机制却一直不清楚。近期发表的3篇论文揭示了外显子拼接复合体(exon junction complexes,EJCs)作为m^(6)A修饰的抑制蛋白suppressors,塑造了m^(6)A表观转录组的形成,解决了这一重大问题。由此,本文简要介绍了m^(6)A修饰通路,并结合这些研究成果阐述了EJC对m^(6)A修饰形成的作用和机理,进而说明外显子-内含子结构通过m^(6)A修饰影响mRNA的稳定性,以期理解m^(6)A这一RNA表观修饰领域的最新进展。
N^(6)-methyladenosine(m^(6)A)is one of the most abundant chemical modifications in mRNA and plays essential roles in diverse physiological and pathological processes.m^(6)A is highly enriched near stop codons and in long internal exons of mRNA,but the mechanism leading to this specific distribution has been unclear.Recently,three papers have solved this major problem by revealing that exon junction complexes(EJCs)act as m^(6)A suppressors and shape the formation of the m^(6)A epitranscriptome.Here,we briefly introduce the m^(6)A pathway,elaborate the roles of EJC on the formation of m^(6)A modification based on these results,and describe the effect of exon-intron structure on mRNA stability via m^(6)A,which will help us better understand the latest progress in the m^(6)A RNA modification field.
作者
宋鹏辉
马丽娟
严冬
Penghui Song;Lijuan Ma;Dong Yan(State Key Laboratory of Genetic Engineering,School of Life Sciences,Fudan University,Shanghai 200438,China;CAS Center for Excellence in Molecular Plant Sciences,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai 200032,China)
出处
《遗传》
CAS
CSCD
北大核心
2023年第6期464-471,共8页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:31970786,32270868)资助。
